左旋肉碱抗疲劳实验研究

目的研究左旋肉碱抗疲劳的效果。方法将健康雄性昆明小鼠80只随机分入四个组：实验前肌糖原和肝糖原测定组、负重游泳组、血乳酸测定组、尿素氮测定组,每个实验各20只小鼠。每个组再随机分成对照组和实验组,每组10只。对照组每天以蒸馏水灌胃,实验组以600mg/kg左旋肉碱灌胃,连续6周后分别测定肝糖原、肌糖原、游泳时间、血乳酸、血清尿素氮含量等指标,观察左旋肉碱抗疲劳效果。结果左旋肉碱组小鼠游泳时间和运动前肌肝糖原含量较对照组明显增加（P〈0.05）,游泳30min和游泳60min的肌肝糖原消耗均较对照组明显减少（P〈0.05）,力竭游泳后肝糖原消耗较对照组显著减少（P〈0.05）,但肌糖原消耗与对照组比较差异无统计学意义（P〉0.05）;运动后左旋肉碱组乳酸增加较对照组明显降低（P〈0.05）,休息30mim后血乳酸含量较对照组降低,恢复率明显增加（P〈0.05）;运动结束休息60min后左旋肉碱组血清尿素氮增量与对照组比较差异无统计学意义（P〉0.05）,但休息120min后,左旋肉碱组血清尿素氮含量较对照组明显降低,恢复率显著增高（P〈0.05）。结论左旋肉碱具有抗疲劳作用,其抗疲劳作用机理主要与左旋肉碱增加肌肝糖原储备,减少运动后肌肝糖原消耗,抑制乳酸生成,加速乳酸和尿素氮清除有关。

Empirical study on anti-fatigue effect of L-Carnitine

Objective To study the anti-fatigue effect of L-Carnitine.Methods 80 KM mice were randomly divided for 4 experimental studies（20 mice /experiment）,namely the hepatic and muscle glycogen test,adopted weight loading swimming test,serum lactic acid test and serum urea nitrogen contents test.In each experiment,the mice were randomly divided into 2 groups（10/group）,namely the control group and the treatment group.Mice in the treatment group were fed with 600 mg/kg of L-Carnitine for 6 weeks,and the mice in the control group were fed with distilled water.Swimming time,hepatic glycogen,muscle glycogen,serum lactic acid,serum urea nitrogen contents were measured.Results The swimming time of the L-Carnitine treatment group was prolonged significantly（P0.05）.Before the swimming test,hepatic and muscle glycogen contents in the treatment group were higher than the control group（P0.05）.The rate of consumption of hepatic glycogen after swimming for 30 minutes and the rate of consumption of hepatic and muscle glycogen afters swimming for 60 minutes in the treatment group were less than the control group（P0.05）.The consumption of hepatic glycogen after exhausted swimming was also decreased remarkably in the treatment group（P0.05）.There was no significant different in the consumption of muscle glycogen between the treatment and control group（P0.05）.In L-Carnitine treatment group,lactic acid content was increased after swimming and the level was lower than the control mice after 30 minutes of resting（P0.05）,and the recovery rate increased remarkably（P0.05）.After 60 minutes of resting,the level of serum urea between L-Carnitine group and the control group was similar（P0.05）.After resting for 120 minutes,the level of serum urea decreased significantly in L-Carnitine treatment group,and the recovery rate was increased significantly（P0.05）.Conclusion L-Carnitine has an anti-fatigue effect.The action mechanism is mainly related to the increase in the reserve of hepatic and muscle glycogen,decreasing consumption of hepatic and muscle glycogen post-exercise,suppression of lactic acid production and accelerating removal of lactic acid and urea nitrogen.