巨细胞病毒感染与儿童免疫性血小板减少性紫癜的关系探讨

目的探讨人巨细胞病毒（HCMV）感染与儿童免疫性血小板减少性紫癜（ITP）的关系。方法采集154例ITP患儿（ITP组）和50例健康儿童（对照组）的血清样本,用Real-time PCR检测HCMV DNA,ELISA方法检测HCMV IgM、IgG抗体;其中105例ITP患儿和50例健康对照儿童采集了尿液标本,用Real-time PCR检测HCMV DNA,并比较ITP组HCMV DNA阳性患儿与阴性患儿的血小板数量的差异。结果 ITP患儿血清HCMV DNA、HCMV IgM及IgG抗体和尿HCMV DNA阳性率均明显高于健康对照儿童,两组比较差异均有统计学意义（P〈0.01）;ITP组HCMV DNA阳性患儿的血小板数量（29.72±14.54）×109/L与阴性患儿（41.28±18.35）×109/L比较差异有统计学意义（P〈0.05）。结论儿童感染HCMV可能是发生ITP的重要致病因素之一,这对指导临床有效治疗及预防ITP的发生有着积极的意义。

Exploration of the relationship between human cytomegalovirus infection and immune thrombocytopenic purpura in children

Objective To explore the relationship between human cytomegalovirus（HCMV） infection and immune thrombocytopenic purpura（ITP） in children.Methods HCMV DNA in serum samples from 154 cases with ITP（ITP groups） and 50 healthy children（control groups） was detected by Real-time PCR.HCMV IgM and HCMV IgG were tested by ELISA simultaneously.Urine specimens from 105 cases in ITP group and 50 children in control group were collected for the detection of HCMV DNA by Real-time PCR.At the same time,amount of platelet（PLT） in HCMV DNA positive children was compared with that of the HCMV DNA negative ones for the ITP groups.Results The positive rates of HCMV DNA,HCMV IgM and HCMV IgG in serum samples in the ITP group were higher than those in the control group,and the HCMV DNA in urine specimens was also found in more cases in the ITP group.There was significant difference between both groups（P0.01）.There were significant difference of PLT between HCMV DNA positive children and the negative ones in the ITP group（P0.05）.Conclusion HCMV infection may be an important pathogenic factor in ITP.This finding is important for the treatment and prevention of ITP.