普通冻伤与高原冻伤血液微循环恢复的差异

目的 探讨普通冻伤与高原冻伤伤口愈合过程中血液微循环恢复的差异.方法 健康雄性SD大鼠24只,随机分为空白对照组、普通冻伤组和高原冻伤组,每组8只.普通冻伤组直接采用液氮渗透法通过控制冷冻时间制作中度冻伤模型.高原冻伤组大鼠缺氧习服1周后,采用与普通冻伤组相同的方法制作高原冻伤模型.建模后第3、7、11、15、19、23天,比较各组伤口面积,然后采用鼠尾静脉注射SonoVue微泡的方法进行超声造影,对伤口血液微循环进行成像,并采用Image Pro软件对微泡浓度进行统计分析,明确微循环恢复情况.结果 在不同时间点,高原冻伤组伤口面积均明显大于普通冻伤组(P<0.05).空白对照组在各时间点微泡浓度均保持在约27×109个/ml水平.而高原冻伤组微泡浓度在伤后不同时间点均明显低于空白对照组和普通冻伤组(P<0.05),普通冻伤组微泡浓度在伤后3、7、11、15、19天明显低于空白对照组(P<0.05),但在伤后第23天与空白对照组比较已无明显差异(P>0.05).结论 高原冻伤伤口血液微循环的恢复速度低于普通冻伤.

Difference in blood microcirculation recovery between normal frostbite and high-altitude frostbite

Objective To determine the difference in blood microcirculation recovery between normal frostbite and high-altitude frostbite during the wound healing.Methods Twenty four male rats were randomly divided into control group (n=8), normal frostbite group (n=8), and high-altitude group (n=8). The normal frostbite group rats were frozen to produce mid-degree frostbite models by controlling the freezing time with liquid nitrogen penetration equipment. The high-altitude frostbite group rats were acclimated to a hypoxic and low-pressure environment for 1 week, and then the high-altitude frostbite models were constructed by the same way with liquid nitrogen penetration apparatus. On days 3, 7, 11, 15, 19, and 23 after modeling, the recovery situation of blood circulation of each group was observed with contrast ultrasonography by injecting SonoVue micro-bubble into rats' tail. Finally, the micro-bubble concentration (MC) was calculated to confirm the blood circulation recovery with software Image Pro. ResultsAt different time points, the wound area of the high-altitude frostbite group was bigger than that of the normal frostbite group, and the MC of control group was always about (27±0.2)×109/ml. On day 3, 7, 11, 15, 19, and 23, the MC was significantly lower in the high-altitude frostbite group than in the control group and normal frostbite group (P<0.05). The MC of normal frostbite group was significantly lower than that of the control group on day 3, 7, 11, 15 and 19 (P<0.05). In addition, no obvious difference in MC was found between normal group and control group on the 23th day (P<0.05).Conclusion The blood microcirculation recovery after high-altitude frostbite is significantly slower than the normal frostbite.