| 用PCR-SSCP及DNA测序法研究胃癌p53基因突变 |
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| 引用本文： | 徐珊,单祥年. 用PCR-SSCP及DNA测序法研究胃癌p53基因突变[J]. 医学争鸣, 2001, 22(14): 1330-1333 |
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| 作者姓名： | 徐珊 单祥年 |
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| 作者单位： | 1. 南京医科大学基础医学院生物学教研室,
2. 南京铁道医学院基础部生物学教研室, |
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| 摘 要： | 目的 探讨p53基因突变与人类胃癌发生的关系。方法 运用PCR、PCR－SSCP结合银染法对25例胃癌组织p53基因第4、第5－6和第7外显子进行突变检测；运用双脱氧链终止法对第7外显子阳性突变标本进行DNA测序。结果 在25例胃癌组织中检出突变5例，突变率为20％；在第7外显子阳性突变标本中发现了18bp的大片段缺失。结论 p53基因突变与人类胃癌的发生具有明显相关性，并且这种突变可能不仅仅局限于点突变，大片段缺失也是p53基因突变方式之一。
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| 关 键 词： | p53基因 突变 胃肿瘤 聚合酶链反应 银染色法 序列分析 DNA |
| 文章编号： | 1000-2790(2001)14-1330-04 |
| 修稿时间： | 2001-03-10 |
A study on mutation of p53 gene in human gastric cancer with methods of PCR-SSCP and DNA sequencing |
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| XU Shan ,SHAN Xiang Nian. A study on mutation of p53 gene in human gastric cancer with methods of PCR-SSCP and DNA sequencing[J]. Negative, 2001, 22(14): 1330-1333 |
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| Authors: | XU Shan SHAN Xiang Nian |
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| Affiliation: | XU Shan 1,SHAN Xiang Nian 2 1Department of Biology,Basic Medical School,Nanjing Medical University,Nanjing 210029,China,2Department of Biology,Faculty of Preclinical Medicine,Nanjing Railway Medical College,Nanjing 210009,China |
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| Abstract: | AIM To investigate the relationship between the mutation of p53 gene and human gastric cancer. METHODS Mutations in exon 4~7 of p53 gene were examined in 25 cases of gastric cancer by PCR and PCR SSCP Silver staining. In addition, Sanger method (dideoxy mediated chain termination method) of DNA sequencing was used to examine the exon 7 which was positive in p53 mutation. RESULTS Mutations were found in 5 cases (20%), and a large fragment deletion of 18 bp was also found in exon 7 which was positive in p53 mutation. CONCLUSION There is a strong correlation between p53 mutation and human gastric cancer. Moreover, the mutation may not be confined to point mutation only. It can be a large fragment deletion. |
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| Keywords: | genes p53 mutation stomach neoplasms polymerase chain reaction silver staining sequence analysis DNA |
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