高效液相色谱法测定接骨胶囊中阿魏酸、补骨脂素及异补骨脂素的含量

 目的建立以高效液相色谱法测定接骨胶囊中阿魏酸、补骨脂素及异补骨脂素含量的方法。方法以甲醇-1%冰醋酸(40∶60)为流动相，于330 nm波长处，以外标法测定。结果阿魏酸在0.25～8.0 μg·mL^-1；补骨脂素在0.75～12.0 μg·mL^-1；异补骨脂素在1.0～16.0 μg·mL^-1范围内，浓度与峰面积线性关系良好(r=0.9999，0.9998及0.9997)。平均回收率为：96.60%，101.1%，99.62%；RSD为0.86%，0.93%，0.74%。结论此种方法灵敏、准确、简便。

Determination of ferulie acid,psoralen and isopsoralen in Jiegu capsule by HPLC

OBJECTIVE To establish a HPLC method for the determination of feulic acid,psoralen and isopsoralen in Jiegu capsule. METHODS The samples were extracted with chloroform by ultrasonic agitation and determined by HPLC using methanol water glacial acetic acid(40∶60) as the mobile phase with UV detection at 330 nm. RESULTS The method was linear over the ragne of 0.25～8.0μg·mL^-1(r＝0.9999) for ferulic acid,0.75～12.0 μg·mL^-1(r=0.9998) for psoralen and 1.0～16.0μg·mL^-1(r=0.9997) for isopsoralen,respectively.The average recoveries were 96.6%,101.1% and 99.62% with 0.86%,0.93% and 0.74% of RSD (n=5),respectively. CONCLUSION The HPLC method was sensitive,simple and accurate.