bcl—2基因在良性前列腺增生中的作用

利用前列腺组织特异性的基因载体新型Ｃ３（１）和转基因技术，培植出人ｂｃｌ２基因的前列腺组织特异性转基因鼠。免疫组织化学检测证实人ｂｃｌ２蛋白在鼠前列腺上皮细胞中的过量表达。病理学观察发现，转基因鼠已发生良性前列腺增生。该实验为说明ｂｃｌ２基因在良性前列腺增生（ＢＰＨ）中的病理作用提供了最直接的证据，并繁殖出可稳定传代的ＢＰＨ的动物模型。

Action of protooncogene bcl 2 in the development of BPH

To establish an animal model for prostate hyperplasia,three lines of transgenic mice were generated by using the prostate specific rat C3(1) promotor to drive expression of the human bcl 2 cDNA. The tissue specific overexpression of human bcl 2 protein in prostatic epithilium was confirmed by immunohistochemical analysis.Male transgenic mice develop atypical benign prostate hyperplasia by 10 months of age in up to 50% of animals. The experiment provides the immediate evidence of the critical role of protooncogene bcl 2 in the development of BPH.Moreover, these transgenic mice offered an suitable animal system to study the etiology of BPH.