PROLIFERATION AND DIFFERENTIATION OF NEURAL STEM CELLS IN ADULT RATS AFTER CEREBRAL INFARCTION |
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Authors: | Bo Zhang Ren-zhi Wang Yong Yao Zhi-hai Liu Zhi-gang Lian Yu-jie Zou Yu-kui Wei |
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Institution: | Department of Neurosurgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730. |
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Abstract: | Objective To investigate proliferation and differentiation of neural stem cells in adult rats after cerebral infarction.Methods Models of cerebral infarction in rats were made and the time-course expression of bromodeoxyuridine (BrdU), Musashil, glial fibrillary acidic protein (GFAP), and neuronal nuclear antigen (NeuN) were determined by immunohistochemistry and immunofluorescence staining. BrdU and Musashil were used to mark dividing neural stem cells. GFAP and NeuN were used to mark differentiating neural stem cells.Results Compared with controls, the number of BrdU-labeled and BrdU-labeled with Musashil-positive cells increased strikingly 1 day after cerebral infarction; approximately 6 fold with a peak 7 days later; markedly decreased 14 days later, but was still elevated compared with that of controls; decling to the control level 28 days later. The number of BrdU-labeled with GFAP-positive cells nearly remained unchanged in the hippocampus after cerebral infarction. The number of BrdU-labeled with NeuN-positive cells increased strikingly 14 days after cerebral infarction, reached maximum peak in the hippocampus 28 days after cerebral infarction in rats.Conclusion Cerebral infarction stimulate proliferation of inherent neural stem cells and most proliferated neural stem cells differentiate into neurons. |
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Keywords: | cerebral infarction neural stem cell proliferation differentiation CEREBRAL INFARCTION ADULT RATS NEURAL STEM CELLS DIFFERENTIATION stimulate differentiate neurons maximum nearly hippocampus control level fold peak number cells increased Results controls used mark |
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