茶多酚抑制人甲状腺乳头状癌细胞生长

目的通过体外细胞培养,观察茶多酚对人甲状腺乳头状癌细胞(CGTHW3)的增殖、侵袭能力及凋亡的影响。方法用MTT法检测细胞增殖活性,观察不同浓度茶多酚对CGTHW3细胞增殖活性的影响;并通过培养板划痕法观察茶多酚对CGTHW3细胞侵袭能力的影响;应用流式细胞仪检测各浓度茶多酚诱导CGTHW3细胞凋亡的作用。结果茶多酚(50～400mg/L)抑制CGTHW3细胞增殖,其作用呈浓度、时间依赖关系。茶多酚对CGTHW3细胞的半量抑制浓度(IC50)为第1天240mg/L,第3天229mg/L,第5天200mg/L,第7天118mg/L。在细胞侵袭实验中,茶多酚可使反映细胞侵袭能力的无细胞带增宽。经茶多酚作用48h后,CGTHW3细胞凋亡百分比明显增加,对照组凋亡百分比为3.51%,100mg/L茶多酚组为12.61%,200mg/L茶多酚组为52.97%,400mg/L茶多酚组为70.79%(P<0.01)。结论茶多酚抑制CGTHW3细胞增殖。茶多酚对CGTHW3细胞的侵袭能力有一定的抑制作用。茶多酚可诱导CGTHW3细胞凋亡。

Inhibitive effect of tea polyphenols on the growth of human thyroid papillary carcinoma cells

Objective To observe the effect of tea polyphenols on proliferation, invasion and apoptosis of human thyroid papillary carcinoma cells (CGTH W-3) in vitro. Methods The effects of various concentrations of tea polyphenols on proliferation, invasion and apoptosis of CGTH W-3 cells were observed by MTT assay, plate scarification assay and flow cytometer, respectively. Results Tea polyphenols(50-400 mg/L) inhibited the proliferation of CGTH W-3 cells with dose-and time-dependent manners. The IC_50 of tea polyphenols to CGTH W-3 cells was 240 mg/L (1st day), 229 mg/L (3 rd day), 200 mg/L (5th day) and 118 mg/L (7th day). The cell free zoster which reflected the invasive ability was widened by tea polyphenols. The apoptosis percentage of CGTH W-3 cells incubated with tea polyphenols for 48 h was obviously increased, with 3.51% in control group, 12.61% with 100 mg/L tea polyphenols, 52.97% with 200 mg/L tea polyphenols and 70.79% with 400 mg/L tea polyphenols (all P<0.01). Conclusion Tea polyphenols inhibit the proliferation of CGTH W-3 cell in vitro. Tea polyphenols have certain inhibitory effect on invasion of CGTH W-3 cell on fibronectin-coated plate and tea polyphenols can induce apoptosis of CGTH W-3 cells.