| 芩术四物汤中黄芩苷和芍药苷的含量测定 |
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| 引用本文： | 黄道秋,贺钢民,李柏群,彭腾,邓赟,邱建平,李鸿翔. 芩术四物汤中黄芩苷和芍药苷的含量测定[J]. 成都中医药大学学报, 2012, 35(3): 45-47 |
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| 作者姓名： | 黄道秋 贺钢民 李柏群 彭腾 邓赟 邱建平 李鸿翔 |
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| 作者单位： | 1.重庆三峡中心医院药剂科,重庆万州,404000;2.成都中医药大学中药材标准化教育部重点实验室,四川成都,611137 |
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| 基金项目： | 重庆市科委科技攻关资助项目(编号:CSTC,2011AC5141) |
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| 摘 要： | 目的:建立芩术四物汤中黄芩苷、芍药苷的含量测定方法,控制其质量.方法:采用Diamonsil C18 (250 mm ×4.6 mm,5m)色谱柱;流动相分别为甲醇-水-磷酸(47∶ 53∶0.2),乙腈-水-磷酸(14∶86∶0.1);检测波长为280 nm及230 nm;流速:1.0 mL/min;柱温:25℃.结果:复方中黄芩苷在0.03147 ～1.007 mg/mL范围内线性关系良好,回归方程为Y=3E-08X-0.0037(r=0.9999),平均回收率为98.17％,RSD％=1.14％.芍药苷在0.03116～0.997 mg/mL范围内线性关系良好,回归方程为Y=8E-0.8
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| 关 键 词： | 芩术四物汤 黄芩苷 芍药苷 高效液相 |
Determination of Baicalin and Paeoniflorin in Qin-zhu-si-wu Decoction |
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| Affiliation: | HUANG Dao-qiu1,HE Gang-min2,LI Bo-qun1,et al(1.Sanxia Central Hospital of Chongqing,Chongqing,404000,China;2.The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine,Chengdu University of TCM,Chengdu,611137,China) |
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| Abstract: | Objective:To establish the assay methods for paeoniflorin and baicalin acid in Qin-Zhu-si-wu Decoction,controlling its quality.Methods:The contents of baicalin and paeoniflorin were analyzed by HPLC.Chromatographic conditions included:Diamonsil C18(250 mm×4.6 mm,5 μm) column and the mobile phase consisted of a mixture of methanol-water-phosphoric acid(47:53:0.2),acetonitrile-water-phosphoric acid(14:86:0.1).Baicalin and paeoniflorin acid were detected at 280 nm and 230 nm respectively,and the flow rate was 1.0 ml/min.Column temperature is 25℃.Results:The linear range was from 0.03147 to 1.007 mg/ml for Baicalin.Regression equation:Y=3E-08X-0.0037(r=0.9999);The average recovery was 98.17 %,RSD=1.14 %.The linear range was from 0.03116 to 0.997mg/ml for Paeoniflorin.Regression equation:Y=8E-0.8X+0.0011(r=1);The average recovery was 97.83%,RSD=1.24%.Conclusion:The method wag simple,rapid,precise and repeatable.This method Can be used for controlling the quality of Qinzhusiwu Decoctio. |
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| Keywords: | Qinzhusiwu Decoctio Baicalin Paeoniflorin HPLC |
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