芩术四物汤中黄芩苷和芍药苷的含量测定

目的:建立芩术四物汤中黄芩苷、芍药苷的含量测定方法,控制其质量。方法:采用Dia-monsil C18(250 mm×4.6 mm,5 m)色谱柱;流动相分别为甲醇-水-磷酸(47∶53∶0.2),乙腈-水-磷酸(14∶86∶0.1);检测波长为280 nm及230 nm;流速:1.0 mL/min;柱温:25℃。结果:复方中黄芩苷在0.03147～1.007 mg/mL范围内线性关系良好,回归方程为Y=3E-08X-0.0037(r=0.9999),平均回收率为98.17%,RSD%=1.14%。芍药苷在0.03116～0.997 mg/mL范围内线性关系良好,回归方程为Y=8E-0.8X+0.0011(r=0.9999),平均回收率为97.83%,RSD%=1.24%。结论:该方法简便、快速、准确、重复性好,为芩术四物汤的质量控制提供了理论依据。

Determination of Baicalin and Paeoniflorin in Qin-zhu-si-wu Decoction

Objective:To establish the assay methods for paeoniflorin and baicalin acid in Qin-Zhu-si-wu Decoction,controlling its quality.Methods:The contents of baicalin and paeoniflorin were analyzed by HPLC.Chromatographic conditions included:Diamonsil C18(250 mm×4.6 mm,5 μm) column and the mobile phase consisted of a mixture of methanol-water-phosphoric acid(47:53:0.2),acetonitrile-water-phosphoric acid(14:86:0.1).Baicalin and paeoniflorin acid were detected at 280 nm and 230 nm respectively,and the flow rate was 1.0 ml/min.Column temperature is 25℃.Results:The linear range was from 0.03147 to 1.007 mg/ml for Baicalin.Regression equation:Y=3E-08X-0.0037(r=0.9999);The average recovery was 98.17 %,RSD=1.14 %.The linear range was from 0.03116 to 0.997mg/ml for Paeoniflorin.Regression equation:Y=8E-0.8X+0.0011(r=1);The average recovery was 97.83%,RSD=1.24%.Conclusion:The method wag simple,rapid,precise and repeatable.This method Can be used for controlling the quality of Qinzhusiwu Decoctio.