胃肠乐对乙酸慢性胃溃疡大鼠HSP70、HSP27表达及溃疡愈合质量的影响

目的探讨胃肠乐(由党参、白术、田七、丹参、香附等组成)促进溃疡修复愈合的效应及其可能的机制.方法48只大鼠随机分为正常对照组、模型对照组及胃肠乐高、中、低剂量组和洛赛克组,复制乙酸慢性胃溃疡模型后给药12 d,计算各组溃疡指数,免疫组化法检测胃粘膜组织热休克蛋白70(HSP70)、热休克蛋白27(HSP27)的表达.结果胃肠乐高、中、低剂量组及洛赛克组胃粘膜溃疡指数均较模型对照组明显下降(P＜0.01).胃肠乐高、中、低剂量组胃粘膜HSP70、HSP27的表达均较模型对照组显著增强(P＜0.05).结论胃肠乐可促进乙酸慢性胃溃疡大鼠溃疡的愈合,提高溃疡愈合质量,此作用可能与其促进胃粘膜组织表达HSP70、HSP27有关.

Effect of Weichangle on Expression of HSP70 and HSP27 and Healing Quality in Rats with Chronic Acetous Gastric Ulcer

Objective To investigate the effect and mechanism of Weichangle consisting of tangshen root, rhizome of largehead atractylodes, root of notoginseng, root of red-rooted salvia, rhizome of nutgrass galingale and other herbs for promoting the healing of ulcer and protecting the gastric mucosa cells. Method To divide randomly 48 SD rats into 6 groups: the normal control group, the model control group, the high-dose Weichangle group, the mid-dose Weichangle group, the low-dose Weichangle group and the Losec (omeprazole) group. The rat model of chronic acetous gastric ulcer (CAGU) were established and treated for 12 days. The ulcer index (UI) was measured by a vernier and the expression of heat shock protein70 (HSP70) and heat shock protein 27 (HSP27) in gastric mucous membrane were detected by immunohistochemistry. Result The UI in three groups of Weichangle and Losec group were significantly lower than those in the model group (P<0.01). The expressions of HSP70 and HSP27 in three groups of Weichangle were obviously higher than those in the model control group (P<0.05). Conclusion Weichangle can promote the healing of chronic acetous gastric ulcer and increase the quality of ulcer healing. The mechanism may be related to its promoting effect on the expression of HSP70 and HSP27.