垂体瘤转化基因、p53蛋白在大肠腺瘤与大肠癌组织中的表达

目的 探讨垂体瘤转化基因(PTTG)和p53蛋白在大肠腺瘤、大肠癌及癌旁正常黏膜组织中的表达及其相关性.方法 采用免疫组化方法检测50例大肠腺瘤和42例大肠癌及癌旁正常黏膜组织中的PTTG、p53蛋白表达水平,并分析两者的相关性以及其与临床病理特征的关系.结果 PTTG在癌旁正常黏膜组织中的阳性表达率为7.14％(3/42),p53蛋白未见阳性表达；PTTG、p53蛋白在大肠腺瘤组织中的阳性表达率分别为82.00％(41/50)和90.00％(45/50),表达强度均未达到过度表达标准；PTTG、p53蛋白在大肠癌组织中的阳性表达率分别为88.10％(37/42)和95.24％( 40/42),其中部分呈过度表达,过度表达率分别为45.24％(19/42)和69.05％( 29/42).PTTG和p53蛋白在大肠癌组织中的阳性表达率显著高于癌旁正常黏膜组织及大肠腺瘤组织,且癌旁正常黏膜组织低于大肠腺瘤组织,差异有统计学意义(P＜ 0.05).在大肠腺瘤组织中,PTTG与p53蛋白的阳性表达密切相关(P＜0.05),但在大肠癌组织中,两者之间的阳性表达无明显相关性(P＞ 0.05).此外,PTTG的过度表达与大肠癌淋巴结转移明显相关(P＜0.01),p53蛋白的过度表达则与大肠癌淋巴结转移无明显相关性(P＞0.05).结论 PTTG、p53蛋白的阳性表达在大肠腺瘤组织中密切相关,两者联合观察可以作为大肠腺瘤癌变的分子指标；PTTG、p53蛋白在大肠癌组织中呈过度表达,PTTG与淋巴结转移有关,PTTG的过度表达可以作为判断大肠癌是否有淋巴结转移的分子指标.

Expression of pituitary tumor transforming gene and p53 protein in large intestinal adenoma and large intestinal carcinoma tissues

Objective To investigate the expression and relationship of pituitary tumor transforming gene (PTTG) and p53 protein in large intestinal adenoma,large intestinal carcinoma and normal large intestinal mucosa tissues.Methods Immunohistochemistry was employed to determine the expression of PTTG and p53 protein in 50 cases with large intestinal adenoma tissues,42 cases with large intestinal carcinoma tissues and normal large intestinal mucosa tissues.The relationship of the expression of PTTG and p53 protein with the clinicopathological characteristics was analyzed.Results There was no positive expression of p53 protein in normal large intestinal mucosa tissues,while the positive rate of PTTG expression was 7.14％(3/42).The positive rates of PTTG and p53 protein expression were 82.00％(41/50) and 90.00％(45/50) in large intestinal adenoma tissues,88.10％ (37/42) and 95.24％ (40/42) in large intestinal carcinoma tissues.The positive rates of PTTG and p53 protein over expression were 45.24％(19/42) and 69.05％(29/42) in large intestinal carcinoma tissues.The positive rates of PTTG and p53 protein expression in large intestinal carcinoma tissues were higher than those in large intestinal adenoma tissues and normal large intestinal mucosa tissues,the positive rates of PTTG and p53 protein expression in large intestinal adenoma tissues were higher than those in normal large intestinal mucosa tissues,and there were significant differences(P ＜ 0.05).The expression of PTTG was not correlated with p53 protein in large intestinal carcinoma tissues(P＞ 0.05 ),while the positive relationship was found between the expression of PTTG and p53 protein in large intestinal adenoma tissues (P ＜ 0.05 ).The over expression of PTTG was significantly correlated with lymph node metastasis (P ＜ 0.01 ),but the over expression of p53 protein was not correlated with lymph node metastasis (P ＞ 0.05) in large intestinal carcinoma tissues.Conclusions The expression of PTTG is significantly correlated with p53 protein in large intestinal adenoma tissues,and their co-expression may be used as markers for carcinogenesis of large intestinal adenoma tissues.The over expression of PTTG and p53protein is found in large intestinal carcinoma,and the over expression of PTTG is correlated with lymph node metastasis.The over expression of PTTG may be used as a marker for lymph node metastasis of large intestinal carcinoma.