磷酸化哺乳动物雷帕霉素靶蛋白在人神经胶质瘤中的表达及临床意义

目的 研究磷酸化哺乳动物雷帕霉素靶蛋白( pmTOR)在人神经胶质瘤中的表达及其与肿瘤抗凋亡和增殖能力的关系.方法 收集人神经胶质瘤石蜡标本87例,其中Ⅰ～Ⅱ级27例、Ⅲ级24例、Ⅳ级36例,应用免疫组织化学EliVision法检测组织中pmTOR、存活蛋白(Survivin)和增殖细胞核抗原(Ki-67)的表达,分析pmTOR与神经胶质瘤病理分级的关系及其与Survivin和Ki-67的相关性.结果 pmTOR在不同级别的神经胶质瘤中均有表达,Ⅰ一Ⅱ级阳性表达率为77.8％(21/27),Ⅲ级为75.0％(18/24),Ⅳ级为72.2％ (26/36),各级别神经胶质瘤中的阳性表达率比较差异无统计学意义(P＞0.05),但其表达强度随着肿瘤病理分级的增高而升高.pmTOR与Survivin和Ki-67的表达强度呈正相关(r=0.858,P＜ 0.01；r=0.708,P＜0.01).结论 pmTOR的表达强度与神经胶质瘤的恶性程度以及细胞抗凋亡和增殖能力有关,可作为基因治疗的有效靶点.

Expression and clinical significance of phosphorylation of mammalian target protein of rapamycin in human glioma

Objective To investigate the association of phosphorylation of mammalian target protein of rapamycin (pmTOR) expression with glioma malignancy grades,and the correlation of pmTOR expression with Survivin and Ki-67,which represent tumor cell anti-apoptosis ability and reproductive activity.Methods Immunohistochemistry EliVision method was employed to detect the expression of pmTOR,Survivin and Ki-67 in paraffin tissues from 87 patients with glioma (grade Ⅰ - Ⅱ 27 cases,grade Ⅲ24 cases and grade Ⅳ 36 cases).The association between positive expression rate,level of pmTOR and malignancy grades,and the correlation of its expression level with Survivin and Ki-67 were further evaluated.Results There was no significant difference in the positive expression rate of pmTOR among grade Ⅰ - Ⅱ (77.8％,21/27),grade Ⅲ (75.0％,18/24) and grade Ⅳ (72.2％,23/36) (P ＞ 0.05).However,the significant association between pmTOR expression level and malignancy grades was observed.The expression from 87 patients with glioma was significantly positively correlated with Survivin and Ki-67 expression level (r =0.858,P ＜ 0.01 ;r =0.708,P ＜ 0.01 ).Conclusions The expression level of pmTOR is associated with malignancy grades,tumor cell anti-apoptosis ability and reproductive activity.pmTOR may be served as a useful marker for predicting the biological behavior of glioma and a useful target for gene therapy.