| 抗苗勒氏管激素(AMH)对离体人卵巢黄素化颗粒细胞干细胞因子(SCF)表达负调控的研究 |
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| 引用本文： | 胡蓉,王飞苗,罗艳,马会明,吴昕. 抗苗勒氏管激素(AMH)对离体人卵巢黄素化颗粒细胞干细胞因子(SCF)表达负调控的研究[J]. 生殖与避孕, 2011, 31(12): 806-810 |
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| 作者姓名： | 胡蓉 王飞苗 罗艳 马会明 吴昕 |
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| 作者单位： | 宁夏医科大学总医院生殖医学中心/省部共建教育部生育力保持重点实验室,银川,750004 |
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| 基金项目： | 宁夏自然科学基金(NZ10115); 宁夏自治区科技攻关项目(kF2010-49); 宁夏高等学校科研项目(2010297); 宁夏医科大学“申博”项目(201049); 宁夏生殖与遗传重点实验室开放课题(SY200804) |
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| 摘 要： | 目的:研究人卵巢黄素化颗粒细胞中抗苗勒氏管激素(AMH)对干细胞因子(SCF)表达的影响。方法:收集15例年龄<35岁因男方因素行体外受精-胚胎移植(IVF-ET)患者的卵巢颗粒细胞,经原代培养,加入不同浓度基因重组人抗苗勒氏管激素(rhAMH),分别于培养的第4日与第6日采用实时定量PCR(RT-PCR)和免疫组织化学检测空白对照组与各实验组SCFmRNA及蛋白的表达。结果:RT-PCR和免疫组织化学均证实人卵巢黄素化颗粒细胞在不同浓度rhAMH干预后,SCFmRNA及蛋白的表达显著减少(P<0.05),其中15 ng/ml rhAMH抑制作用最明显;实验组第4日与第6日颗粒细胞表达SCFmRNA及蛋白无明显差异(P>0.05)。结论:AMH可有效抑制人卵巢黄素化颗粒细胞SCF mRNA及蛋白的表达。
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| 关 键 词： | 抗苗勒氏管激素(AMH) 颗粒细胞 干细胞因子(SCF) |
Impact of Anti Miillerian Hormone(AMH) on the Expression of Stem Cell Factor (SCF) in Human Granulosa Cells Cultured in Vitro |
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| Rong HU , Fei-miao WANG , Yan LUO , Hui-ming MA , Xing WU. Impact of Anti Miillerian Hormone(AMH) on the Expression of Stem Cell Factor (SCF) in Human Granulosa Cells Cultured in Vitro[J]. Reproduction and Contraception, 2011, 31(12): 806-810 |
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| Authors: | Rong HU Fei-miao WANG Yan LUO Hui-ming MA Xing WU |
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| Affiliation: | Rong HU,Fei-miao WANG,Yan LUO,Hui-ming MA,Xing WU(Dept.of Reproductive Medicine Center of the Affiliated Hospital,Ningxia Medical College/Key Laboratory of Fertility Preservation and Maintenance,Ministry of Education,Yinchuan,750004) |
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| Abstract: | Objective: To explore the influence of anti Mullerian hormone(AMH) on the expression of SCF in human granulosa cells cultured in vitro.Methods: Fifteen patients were recruited who received in vitro fertilization and embryo transfer(IVF-ET) at age < 35 years only because of male sterility,and the granulosa cells were cultured with different concentrations of rhAMH.The expressions of SCF mRNA and protein in granulosa cells were determined by RT-PCR and immunohistochemistry on day 4 and day 6,respectively.Resu... |
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| Keywords: | anti Mūlerian hormone（AMH） human granulosa cells stem cell factor（SCF） |
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