牛蒡子苷元对人食管癌细胞增殖、凋亡的影响

目的 观察牛蒡子苷元(ARG)对人食管癌细胞(EC-1)增殖、凋亡的影响,并探讨其机制.方法 将不同浓度的ARG作用于EC-1细胞,采用噻唑蓝(MTT)实验检测细胞增殖活性(ARG浓度分别为2.5、5.0、10.0、20.0、40.0 mg/L,作用时间为24、48、72、96、120 h),流式细胞术检测细胞凋亡率,琼脂糖凝胶电泳和DNA缺口末端标记法(TUNEL)检测细胞凋亡指数,免疫细胞化学法检测凋亡相关基因Bcl-2、Bax表达水平的改变(该3种检测均采用:ARG浓度10.0、20.0、40.0 mg/L,作用时间24h)；分析不同浓度ARG对EC-1细胞的影响.并设阴性对照组(只加培养液不加药物)进行比较.结果 ARG对EC-1细胞增殖、凋亡的影响呈剂量/时间依赖性.随ARG浓度和作用时间的递增,对EC-1细胞增殖的抑制率递升(P＜0.05或P＜0.01).随ARG浓度的递增,EC-1细胞凋亡率和凋亡指数递升(P均＜0.01),Bcl-2表达率递降(P＜0.01),Bax表达率递升(P＜0.01).上述变化与对照组比较,差异均有统计学意义(P＜0.05或P＜0.01).结论 ARG可明显抑制EC-1细胞增殖并诱导其凋亡,其机制可能与ARG调控Bcl-2、Bax基因表达而诱导EC-1细胞凋亡有关.

The effect of arctigenin on proliferation and apoptosis of human esophageal cancer cells

Objective To evaluate the effect of arctigenin(ARG) on proliferation and apoptosis of human esophageal cancer-1(EC-1)cells and explore its mechanism.Methods The proliferation activity of EC-1 cells was detected by methylthiazol tetrazolium(MTT) method in ARG concentration of 2.5,5.0,10.0,20.0,40.0 mg/L and acting time of 24,48,72,96 and 120h.In ARG concentration of 10.0,20.0 and 40.0 mg/L and acting time of 24h,the apoptosis rate of EC-1 cells was assayed by flow cytometry(FCM),and the apoptotic index was respectively measured by agarose gel electrophoresis and TdT-mediated dUTP nick end labeling(TUNEL),and the expressions of apoptosis-related gene Bcl-2 and Bax were respectively detected by immunocytochemistry method.The impact of ARG of different concentration on EC-1 cells was analyzed.The negative control group without ARG was designed for serving as comparison.Results The proliferative activity of EC-1 cells can be inhibited by ARG in a dose/time dependent manner.The proliferation inhibitory rate of EC-1 cells was gradually increased in proportion to the increase of ARG concentration and active time(P<0.05 or P<0.01).The apoptotic rate and apoptotic index of EC-1 cells gradually increased,and the expression rate of Bcl-2 gradually decreased while the expression rate of Bax gradually increased in proportion to the increase of ARG concentration(all P<0.01).There were significant differences on aforementioned changes compared with negative control group(P<0.05 or P<0.01).Conclusions ARG can inhibit the proliferation of EC-1 cells and induce apoptosis of EC-1 cells,and its mechanism may be associated with regulating expressions of Bcl-2 and Bax genes and thus inducing apoptosis of EC-1 cells.