| FISH与IHC技术检测乳腺癌Her2状态的对比研究 |
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| 引用本文： | 赵东晖,卢义生,何建芳,冼丽英. FISH与IHC技术检测乳腺癌Her2状态的对比研究[J]. 实用全科医学, 2010, 8(1): 8-10,F0003 |
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| 作者姓名： | 赵东晖 卢义生 何建芳 冼丽英 |
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| 作者单位： | 广东省东莞市人民医院病理科,523018 |
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| 基金项目： | 卫生部科研基金，东莞市科技计划项目 |
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| 摘 要： | 目的比较荧光原位杂交法（FISH）与免疫组化法（IHC）检测乳腺癌组织中Her2基因扩增和蛋白表达的差异性和相关性。方法IHC法检测56例乳腺癌细胞膜上Her2蛋白表达情况，FISH法检测细胞核内Her2基因扩增情况，Х^2检验及kappa检验对两种方法检测结果进行统计分析。结果两种方法的检测Her2阳性率差异有统计学意义（Х^2=39．867，P〈0．05）；IHC法检测Her2蛋白0～1＋与3＋之间，通过FISH法检测Her2基因扩增的一致性好（k=0．906，P〈0．05）；而IHC检测Her2蛋白2＋与3＋之间，FISH检测Her2基因扩增一致性较差（k=0．357。P〈0．05）。结论IHC检测Her2蛋白为2＋的需做FISH检测，以明确Her2基因扩增的情况，FISH检测法提高了诊断的准确性和特异性。
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| 关 键 词： | 荧光原位杂交 免疫组织化学 乳腺癌 Her2基因 受体 |
Comparative Study on Fluorescence in Situ Hybridization and Immunohistochemistry for Her2 Gene Determine in Breast Cancer |
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| ZHAO Dong-hui,LU Yi-sheng,HE Jian-fang,et al.. Comparative Study on Fluorescence in Situ Hybridization and Immunohistochemistry for Her2 Gene Determine in Breast Cancer[J]. Applied Journal Of General Practice, 2010, 8(1): 8-10,F0003 |
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| Authors: | ZHAO Dong-hui LU Yi-sheng HE Jian-fang et al. |
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| Affiliation: | ZHAO Dong-hui,LU Yi-sheng,HE Jian-fang,et al.Department of Pathology,Dongguan People's Hospital,Dongguan 523018,Guangdong,China |
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| Abstract: | Objective To compare the differences and correlation between fluorescence in situ hybridization(FISH) and immunohistochemistry(IHC) in detection of Her2 gene amplification and expression status in breast cancer tissues.Methods In 56 samples,IHC technique was applied to detect the Her2 protein in the cytomembrane of breast cancer cells,and FISH was used to assess the amplification of Her2 gene,chi-square and kappa test were employed to analyze the relationship between these two methods.Results Significant di... |
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| Keywords: | Fluorescence in situ hybridization(FISH) Immunohistochemistry Breast cancer Her2 gene Receptor |
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