Fast interferometric second harmonic generation microscopy |
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| Authors: | Stéphane Bancelin Charles-André Couture Katherine Légaré Maxime Pinsard Maxime Rivard Cameron Brown Fran?ois Légaré |
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| Affiliation: | 1Institut National de la Recherche Scientifique, Centre Énergie Matériaux Télécommunications (INRS-EMT); 1650 Boul. Lionel-Boulet, Varennes (QC), J3X 1S2, Canada;2University of Oxford, Botnar Research Center, NDORMS, Windmill Road, Oxford, OX3 7HE, UK |
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| Abstract: | We report the implementation of fast Interferometric Second Harmonic Generation (I-SHG) microscopy to study the polarity of non-centrosymmetric structures in biological tissues. Using a sample quartz plate, we calibrate the spatially varying phase shift introduced by the laser scanning system. Compensating this phase shift allows us to retrieve the correct phase distribution in periodically poled lithium niobate, used as a model sample. Finally, we used fast interferometric second harmonic generation microscopy to acquire phase images in tendon. Our results show that the method exposed here, using a laser scanning system, allows to recover the polarity of collagen fibrils, similarly to standard I-SHG (using a sample scanning system), but with an imaging time about 40 times shorter.OCIS codes: (180.4315) Nonlinear microscopy, (190.2620) Harmonic generation and mixing, (170.6935) Tissue characterization, (190.4180) Multiphoton processes, (190.4710) Optical nonlinearities in organic materials |
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