脑脊液净化治疗仪对模拟血性脑脊液体外净化效果的评价

目的 利用脑脊液净化治疗仪对模拟血性脑脊液进行体外净化试验，评价该净化仪的稳定性、滤过效率与滤过承载量，为进一步临床试验提供数据。方法 选择健康人悬浮红细胞及生理盐水配制成2.5%、5%、7.5%的模拟血性脑脊液，脑脊液净化仪及配套的管路系统采用自行研制并获国家专利的产品，将此种模拟血性脑脊液按照未来人体试验的模式进行脑脊液净化仪的体外净化试验，净化参数设置参照正常人体脑脊液循环的生理指标，分析净化前后红细胞计数的变化。结果 在滤膜最大承载试验中，2.5%模拟血性脑脊液的红细胞去除率达到90%以上时需要的循环总量达到330 ml，而5%、7.5%模拟血性脑脊液所需要的循环总量分别为390 ml、420 ml。在红细胞去除率达95%以上时，2.5%、5%、7.5%模拟血性脑脊液所需循环总量分别为390 ml、450 ml、480 ml。在体外模拟人体环境的净化效果试验中，2.5%模拟血性脑脊液单套滤膜净化循环6次后红细胞平均数由153 000个/mm3，下降到63 900个/mm3，下降率超过55%。结论 脑脊液净化仪能够有效去除模拟血性脑脊液中的红细胞，设备的稳定性高。

Evaluation of Red Blood Cell Removing Efficiency of Cerebrospinal Fluid Purification System on Simulative Blooded Cerebrospinal Fluid

Objective To evaluate the stability, efficiency and load capacity of cerebrospinal fluid （CSF） purification system and to provide experimental data for further clinical trial through the purification experiment for simulative blooded cerebrospinal fluid in vitro. Methods 2.5%, 5% and 7.5% simulative blooded CSF were prepared using physiological saline with preserved red blood cells from healthy volunteers. CSF purification system was developed with national patent. According to the purification parameters of normal CSF circulation in vivo, those simulative blooded CSF were purified in vitro using CSF purification system. The number of red blood cells was analyzed before and after purification. Results In the experiment to determine the maximum load capacity of filter membrane, 330ml of fluid circulation were needed in order to achieve more than 90% red blood cell （RBC） removing rate for 2.5% simulative blooded CSF, and 390ml, 420ml were needed separately, for 5% and 7.5%. For more than 95% RBC removing rate, 390ml, 450ml and 480ml of fluid circulation were required for 2.5%, 5% and 7.5%, separately. By six-round purification circulation using a single set of filter membrane, RBC counts in 2.5% simulative blooded CSF decreased to 63 900/mm3 from 153 000/ mm3 and the decline rate exceeded 55%. Conclusion CSF purification system is able to remove RBC effectively from simulative blooded CSF with high stability.