3种灵芝子实体腺苷TLC鉴定及HPLC含量测定

目的建立灵芝属药材赤芝、紫芝和树舌子实体中腺苷的HPLC含量测定及TLC鉴别的方法。方法采用硅胶GF254薄层板,以正丁醇-乙腈-0.1 mol.L-1醋酸铵-浓氨水(6:1:2:1)为展开剂,紫外光灯(254 nm)检视进行腺苷TLC鉴别;采用ZORBAX SB-C1(84.6×250 mm)色谱柱,以甲醇-0.01mo.lL-1磷酸二氢钾(10:90)为流动相,流速1.0 mL.min-1,检测波长:254 nm,柱温26℃为条件进行腺苷HPLC含量测定;结果 TLC法均可从赤芝、紫芝、树舌子实体中鉴别出腺苷;应用HPLC方法测定腺苷在3.125⁵0μg内线性关系良好(r=0.999 3),重复性良好(RSD=1.2%),平均加样回收率为103.9%,精密度良好(RSD=1.1%),24 h内测定结果稳定(RSD=1.1%),测得赤芝、紫芝和树舌的腺苷含量分别为38.7μg/g、35.3μg/g和75.2μg/g。结论采用TLC法和HPLC法鉴定灵芝药材中腺苷,简便易行,重复性好,可作为灵芝属药材(子实体)质量控制另一指标。

Quantitative Determination by HPLC and Identification by TLC of Adenosine in Sporophore of Three Ganoderma lucidum

Objecive To establish methods for identification and determination of adenosine in sporophore of three Ganoderma lucidum named Ganoderma lucidum,Ganoderma sinense Zhao and Ganoderma applanatum.Methods TLC,the stational phase was GF254,the developing solvent consisted of N-ButyL-Acetonitrile: Acetonitrile: 0.1 mol.L-1Ammonium Acetate : Ammonia Solution=6:1:2:1;HPLC,The quantity was performed with Agilent ZORBAX SB-C18 column(4.6×250mm),and the mobile phase was consisted of 10% methanol and 90% 0.01 mol L-1potassium dihydrogen phosphate.The flow rate was 1.0 mL min-1 and the detection wavelength was 254 nm.The detection temperature was 26℃.Results The adenosine in Ganoderma lucidum,Ganoderma sinense Zhao and Gnoderma applanatum was detected by TLC.Adenosine showed good linear in the range of 3.125~50 μg(r = 0.999 3),and the reproducibility was 1.2%.The average recovery was 103.7%.The RSD of precision was 1.1%.The sample solution was stable within 24 h.The contents of adenosine in Ganoderma lucidum,Ganoderma sinense Zhao and Ganoderma applanatum were 38.7μg/g,35.3 μg/g,75.2μg/g,respectively.Conclusion The method is specific,accurate and reproducible,and can be used to control the quality of Ganoderma.