| 肝细胞内HBV DNA原位杂交的研究 |
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| 引用本文： | 张永源
,鄢璞
,沈汉馨
,李淋
,汪由坤
,石淑仙
,宋佩辉
,郝连杰.肝细胞内HBV DNA原位杂交的研究[J].华中科技大学学报(医学版),1990(3). |
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| 作者姓名： | 张永源 鄢璞 沈汉馨 李淋 汪由坤 石淑仙 宋佩辉 郝连杰 |
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| 作者单位： | 同济医科大学附属同济医院临床免疫研究室，同济医科大学附属同济医院传染病学教研室 |
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| 基金项目： | “七五”期间国家重点科技攻关项目 |
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| 摘 要： | 应用生物素标记HBV DNA(乙肝病毒脱氧核糖核酸)作探针,对129例肝病患者肝组织作原位杂交研究。HBV DNA在慢性活动性肝炎中检出率最高(80.7％),显著高于肝硬化、慢性小叶性肝炎、急性肝炎及原发性肝癌组。具有乙肝复制指标阳性患者的肝细胞内HBV DNA检出率(82.0％)明显高于非复制组(63.0％),P<0.05。本研究观察到;HBB DNA阳性肝细胞与肝细胞坏死灶关系密切,多位于肝细胞坏死灶中间或(和)周边,其中以局灶型分布的HBVDNA阳性肝细胞与肝细胞坏死灶关系密切。提示HBV复制与肝细胞坏死有关。
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| 关 键 词： | 乙肝病毒脱氧核糖核酸(HBV DNA) 原位分子杂交 病毒复制 生物素标记探针 |
Morphology,Distribution and Its Significance of Intrahepatic HBV DNA in Liver Disease:a Preliminary Study by in Situ Hybridization |
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| Zhang Yongyuan,Yan Pu,Sheng Hanxin,Li Lin,Wang Youkun,Shi Shuxian,Song Peihui,Hao Lianjie Clinical Immunology Research Unit.Morphology,Distribution and Its Significance of Intrahepatic HBV DNA in Liver Disease:a Preliminary Study by in Situ Hybridization[J].Journal of Huazhong University of Science and Technology(Health Sciences),1990(3). |
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| Authors: | Zhang Yongyuan Yan Pu Sheng Hanxin Li Lin Wang Youkun Shi Shuxian Song Peihui Hao Lianjie Clinical Immunology Research Unit |
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| Institution: | Zhang Yongyuan,Yan Pu,Sheng Hanxin,Li Lin,Wang Youkun,Shi Shuxian,Song Peihui,Hao Lianjie Clinical Immunology Research Unit,Department of Infectious Diseases,Tongji Hospital,Tongji Medical University,Wuhan |
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| Abstract: | A biotin-labelled DNA specific for hepatitis B virus (HBV) nucleo-tide sequences was hybridized in situ to liver tissue of 129 cases with liver diseases.It was found that chronic active hepatitis (CAH) group had a significant higher prevalence (80.7%),as compared with cirrhosis,chronic lobular hepatitis (CLH),acute hepatitis and hepatocellular carcinoma (HCC) groups.HBV DNA in HBeAg positive or intrahepatic HBcAg positive patients (82.0%) was more easily detected than the single HBsAg positive and anti-HBc cases (63.0%,P<0.05).The interesting findings that expressing HBV DNA hepatocytes,particularly as focal distribution were closedly related to hepatic necrotic sites,suggest that HBV replication may occur in conjunction with hepatic necrosis. |
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| Keywords: | HBV DNA in situ hybridization virus replication biotin-probe |
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