| 内皮素1对喉癌细胞HEP 2的促增殖作用 |
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| 引用本文： | 徐定远,郑雪莲,吴波,薛飞,李泽卿,季俊峰,王秋萍. 内皮素1对喉癌细胞HEP 2的促增殖作用[J]. 山东大学耳鼻喉眼学报, 2009, 23(2): 1-4 |
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| 作者姓名： | 徐定远 郑雪莲 吴波 薛飞 李泽卿 季俊峰 王秋萍 |
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| 作者单位： | 徐定远,薛飞,李泽卿,季俊峰,王秋萍,XU Ding-yuan,XUE Fei,LI Ze-qinq,JI Jun-feng,WANG Qiu-ping(南京军区总医院耳鼻咽喉科,南京,210002);郑雪莲,ZHENG Xue-lian(四川大学华西第二附属医院分子与转化实验室,成都,610041);吴波,WU Bo(南京军区总医院病理科,南京,210002) |
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| 基金项目： | 中国博士后基金研究项目，四川省科技厅应用基础研究项目 |
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| 摘 要： | 目的探讨内皮素1对HEP 2细胞有丝分裂的影响。方法采用RT PCR及免疫组化Dako EnVision法检测HEP 2细胞内皮素受体表达情况;3H 胸腺嘧啶核苷渗入法检测不同浓度和作用时间内皮素1对HEP 2细胞有丝分裂的影响。结果HEP 2细胞存在内皮素受体基因及蛋白表达;内皮素1具有明显促HEP2细胞增殖作用,且其促增殖作用呈浓度依赖性。结论内皮素1能有效促进HEP 2细胞有丝分裂,对喉癌的演进可能有重要调控作用。
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| 关 键 词： | 关键词:内皮素 1 HEP 2 细胞 有丝分裂 |
Effect of endothelin 1 on mitosis of human HEP 2 cells in vitro |
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| XU Ding-yuan,ZHENG Xue-lian,WU Bo,XUE Fei,LI Ze-qinq,JI Jun-feng,WANG Qiu-ping. Effect of endothelin 1 on mitosis of human HEP 2 cells in vitro[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2009, 23(2): 1-4 |
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| Authors: | XU Ding-yuan ZHENG Xue-lian WU Bo XUE Fei LI Ze-qinq JI Jun-feng WANG Qiu-ping |
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| Affiliation: | (1. Department of Otorhinolaryngology, Nanjing General Hospital of Nanjing Military Command, Chinese PLA, Nanjing 210002, China; 2. Molecular and Translational Medicine Lab, Second Affiliated Hospital of Sichuan University, Chengdu 610041, China;3.Department of Pathology, Nanjing General Hospital of Nanjing Military Command, Chinese PLA, Nanjing 210002, China) |
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| Abstract: | To investigate the effect of endothelin 1 on mitosis of HEP 2 cells. MethodsExpression of endothelin receptors on HEP 2 cells was determined by RT PCR and immunohistochemical methods. HEP 2 cell lines were exposed to different concentrations of the endothelin 1, and mitosis of HEP 2 cells was measured by 3ResultsGene expression of the endothelinreceptor was detected by RT PCR. Immunohistochemical studies showed that endothelin receptor immunoreactivity appeared in HEP 2 cells. Addition of endothelin 1 resulted in an increase of DNA synthesis in concentration dependence. ConclusionEndothelin 1 can promote mitosis of HEP 2 cells and may play an important role in the progression of laryngeal carcinoma. |
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| Keywords: | Endothelin 1 HEP 2 cells Mitosis |
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