去除血型抗体制备O型红细胞的研究

目的建立去除血浆中血型抗体的O型红细胞的制备工艺,为临床提供安全的O型红细胞制剂。方法使用处理后的冰冻干燥A型、B型红细胞膜,分别加入O型红细胞血浆中吸附血浆血型抗体物质,并检测吸附后红细胞的结构和功能。结果使用海藻糖冻干保存红细胞膜可保存其抗原性,吸附后,可去除血浆中抗A、抗B抗体80%—99%,同时红细胞变形指数、ATP、渗透脆性、上清游离Hb含量与吸附前(正常红细胞)比较差异无统计学意义(P>0.05),均在正常参考范围;流式结果显示,吸附后采用离心方法,红细胞膜去除率可达到77%。结论用冰冻干燥红细胞膜去除ABO血型抗体为O型红细胞的他型输注奠定了基础。

Preparation of group O red blood cells by removing ABO antibodies

Objective To develop a procedure for preparation of group O red blood cell(RBC) suspension by removing anti-A and anti-B in the plasma,and to provide safe group O RBC suspension for clinical use.Methods Lyophilized RBC membrane of group A and B red cells were used to remove the anti-A and anti-B antibodies in the plasma of group O RBCs,and its effects on RBC were observed subsequently.Results The antigenicity of RBC membrane can be well retained by trehalose when RBC membrane was lyophilized.The anti-A and anti-B antibodies in the plasma were removed by 80%—99%.The quality indexes of RBCs,such as morphology,deformability,ATP contents,osmotic fragility,and the free Hb in the supernatant,were within normal range,and showed no significant difference when compared with those of normal RBCs(P>0.05).Flow cytometry data showed that after centrifuge,the clearance rate of added lyophilized red cell membrane was 77%.Conclusion The transfusion of antibody-removed group O RBCs to non-group O patients still needs further research.