波长切换HPLC法快速测定柴银口服液中绿原酸、葛根素和黄芩苷的含量

目的:建立同时测定柴银口服液中绿原酸、葛根素和黄芩苷含量的方法。方法:采用高效液相色谱(HPLC)法。色谱柱为Purospher?STAR RP-18 endcapped(55 mm×4 mm,3μm),流动相为甲醇-2%冰醋酸水溶液(梯度洗脱),检测波长为327 nm(0^<4 min)、250 nm(4<4 min)、250 nm(4^<6 min)、280 nm(6<6 min)、280 nm(6¹0 min),流速为1.0 ml/min,柱温为30℃。结果:绿原酸、葛根素和黄芩苷的质量浓度分别在0.436 810 min),流速为1.0 ml/min,柱温为30℃。结果:绿原酸、葛根素和黄芩苷的质量浓度分别在0.436 8⁸.736 0μg/ml、0.206 48.736 0μg/ml、0.206 4⁴.128 0μg/ml和2.0604.128 0μg/ml和2.060⁴1.200μg/ml范围内与各自峰面积积分值呈良好的线性关系(r均为0.999 9);三者精密度、稳定性、重复性试验的RSD<3%;平均加样回收率分别为99.57%、99.91%和100.08%,RSD分别为1.75%、1.69%和2.20%(n均为6)。结论:波长切换HPLC法简便、快速、准确、可靠,可用于柴银口服液质量的全面控制与评价。

Content Determination of Chlorgenic Acid,Puerarin and Baicalin in Chaiyin Oral Solution by HPLC with Switching Wavelength

OBJECTIVE：To establish the method for the content determination of chlorgenic acid,puerarin and baicalin in Chaiyin oral solution. METHODS：HPLC method was adopted. The determination was performed on Purospher？STAR RP-18 endcapped（55 mm×4 mm,3 μm）column with mobile phase consisted of methanol-2% glacial acetic acid（gradient elution）at the flow rate of 1.0 ml/min. The detection wavelength was set at 327 nm（0-4 min）,250 nm（4-6 min）and 280 nm（6-10 min）. The column temperature was set at 30 ℃. RESULTS：The liner ranges of chlorgenic acid,puerarin and baicalin were 0.4368-8.736 0 μg/ml（r=0.999 9）,0.206 4-4.128 0 μg/ml（r=0.999 9）and 2.060-41.200 μg/ml（r=0.999 9）,respectively. RSDs of precision,stability and reproducibility tests were all lower than 3%. Average recoveries were 99.57%（RSD=1.75%,n=6）,99.91%（RSD=1.69%,n=6）and 100.08%（RSD=2.20%,n=6）,respectively. CONCLUSIONS：The method is simple,rapid,accurate and reliable,and it could be used for the quality control and evaluation of Chaiyin oral solution.