锦灯笼提取物对抗菌药物致小鼠肠道菌群失调的预防作用

目的:研究锦灯笼提取物对抗菌药物致小鼠肠道菌群失调的预防作用。方法:48只BALB/c小鼠随机均分为正常对照(等容生理盐水)组、模型(等容生理盐水)组、丽珠肠乐(2 mg/kg)组与锦灯笼提取物高、中、低剂量(80、40、20 mg/kg)组,灌胃给药,每天1次,连续7 d。末次给药后第2天灌胃盐酸左氧氟沙星(1.3 mg/kg),每天1次,连续7 d以致小鼠肠道菌群失调。收集小鼠粪便,提取基因组DNA,采用聚合酶链反应-变性梯度凝胶电泳(PCR-DGGE)法获得肠道菌群指纹图谱,进行相似性及优势条带的序列分析。结果:灌胃抗菌药物前,小鼠肠道优势菌型为内普雷沃氏菌和乳酸杆菌,高、中、低剂量锦灯笼提取物可增加其乳酸杆菌数量;灌胃抗菌药物后,高、中、低剂量锦灯笼提取物可促进模型小鼠肠道内拟杆菌的生长,使其成为优势菌型。结论:锦灯笼提取物能增加肠道乳酸杆菌的数量,对抗菌药物致小鼠肠道菌群失调有预防作用。

Prevention Effects of Physalis alkekengi Extract against Antibiotic-induced Intestinal Microflora Imbalance of Mice

OBJECTIVE：To study the prevention effect of Physalis alkekengi extracts against antibiotic-induced intestinal microflora imbalance of mice. METHODS：Mice were given levofloxacin hydrochloride intragastrically on the second day after last medication,once a day,for consecutive 7 days. 48 BALB/c mice were randomly divided into normal control group,model group,Lizhu changle group（2 mg/kg）and P. alkekengi high-dose,medium-dose and medium-dose groups（80,40,20 mg/kg）;they were given relevant medicine intragastrically once a day for consecutive 7 days. Then the feces of mice were collected. After extraction of bacterial genomic DNA from feces,the fingerprint of intestinal microflora was determined by PCR-DGGE,and the similarity and the sequence analysis of the dominant bands were carried out. RESULTS：Before intragastric administration of antibiotics,Prevotella and Lactobacillus were dominant organisms;the number of Bacillus acidi lactici was increased by high-dose,medium-dose and low-dose P. alkekengi extract. After intragastric administration of antibiotics,high-dose,medium-dose and low-dose P. alkekengi extract could promote the generation of bacteroid which became dominant organism. CONCLUSIONS：P. alkekengi extract increase the number of Bacillus acidi lactici,and prevent antibiotic-induced intestinal microflora imbalance.