秦皮挥发油成分的GC-MS分析

目的:为秦皮药材的进一步研究和开发提供科学依据。方法:利用水蒸气蒸馏法提取秦皮挥发油,采用气相色谱-质谱(GC-MS)联用技术对挥发油成分进行分析鉴定,用峰面积归一化法计算各成分的质量分数。结果:共分离出65个化学成分,鉴别出61个化学成分,占挥发油总量的93.968%。其中,质量分数较大的成分有4-己基-2,5-二氧代呋喃-3-乙酸(10.197%)、3-氟-4-甲氧基苯胺(9.846%)、葡萄螺烷(7.252%)、1-十五烯(7.228%)、右旋橙花叔醇(6.501%)、氧化芳樟醇(5.465%)、α-红没药醇(4.959%)、反-氧化芳樟醇(4.582%)、4-萜烯醇(3.203%)、柠檬烯(2.134%)、正癸酸(2.019%)。结论:GC-MS联用技术可快速、准确地鉴别秦皮挥发油成分,为秦皮资源的综合开发利用提供了基础数据。

GC-MS Analysis of Volatile Oil from Cortex Fraxini

OBJECTIVE：To provide scientific evidence for further RD of Cortex Fraxini. METHODS：The volatile oil was extracted from Cortex Fraxini by steam distillation. The volatile oil was analyzed by GC-MS and the mass fraction of each component was calculated by normalization method. RESULTS：65 chemical compounds were separated and 61 of them were identified,which accounted for 93.97% of all the volatile oil. The major components were 4-hexyl-2,5-dioxo furan-3-acetic acid（10.197）,3-fluoro-4-methoxyaniline（9.846）,vitis-pirane（7.252）,1-pentadecene（7.228%）,D-nerolidol（6.501%）,linalyl oxid（5.465%）,α-bisabolo（4.959%）,trans-linalool oxide（4.582%）,4- terpenol（3.203%）,limonene（2.134%）and n-decanoic acid（2.019%）.CONCLUSIONS：The volatile oils of Cortex Fraxini can be accurately identified by GC-MS rapidly,which provide basic data for comprehensive utilization of Cortex Fraxini.