芦丁对高糖模型大鼠肾小球系膜细胞的保护作用

目的:研究芦丁对高糖模型大鼠肾小球系膜细胞的保护作用。方法:以高糖(25 mmol/L)培养鼠肾小球系膜细胞。试验随机均分为正常对照(正常培养基)组、溶剂对照(0.1%DMSO)组、模型(正常培养基)组、卡托普利(1μmol/L)组与芦丁高、中、低质量浓度(40、10、5μg/ml)组,培养48 h。采用实时荧光定量-聚合酶链反应(RT-PCR)法测定细胞转化生长因子(TGF)-β1mRNA的表达,Western blot法检测细胞Smad 2/3与Smad 7的含量,分光光度法测细胞总超氧化物歧化酶(T-SOD)、丙二醛(MDA)、过氧化氢酶(CAT)与谷胱甘肽过氧化酶(GSH-Px)水平。结果:与溶剂对照组比较,模型组细胞Smad 2/3、TGF-β1mRNA的表达增强,T-SOD、CAT与GSH-Px活性减弱,MDA含量增加,Smad 7的表达减弱,差异有统计学意义(P<0.01)。与模型组比较,芦丁高、中、低质量浓度组细胞Smad 2/3表达减弱,T-SOD、CAT活性增强,MDA含量减少,Smad 7的表达减弱;芦丁高、中质量浓度组TGF-β1mRNA表达减弱,GSH-Px活性增强,差异有统计学意义(P<0.01或P<0.05)。结论:芦丁通过TGF-β1/Smad通路对高糖培养的大鼠肾小球系膜细胞产生保护作用,具有潜在的防治糖尿病肾病作用。

Protective Effects of Rutin on Mesangial Cells of High Glucose Model Rats

OBJECTIVE：To study the protective effect of rutin on mesangial cells of high glucose model rats. METHODS：The mesangial cells of rats were cultured with high glucose. The cultured mesangial cells were randomly divided into normal control group（normal culture medium）,solvent control group（0.1% DMSO）,model group（normal culture medium）,captopril group（1 μmol/L）and rutin high-concentration,medium-concentration and low-concentration groups（40,10,5 μg/ml）,for culturing 48 h. The mRNA expression of TGF-β1in mesangial cells was detected by RT-PCR;the contents of Smad2/3 and Smad 7 were determined by Western blot;the levels of T-SOD,MDA,CAT,and GSH-Px were measured by spectrophotometry. RESULTS：Compared with solvent control group,the mRNA expression of Smad 2/3 and TGF-β1and MDA content were increased in model group,while the activities of T-SOD,CAT and GSH-Px and the expression of Smad 7 were decreased;there was statistical significance（P〈0.01）. Compared with model group,the expression of Smad 2/3 Smad 7,and MDA content were decreased in rutin high-concentration,medium-concentration and low-concentration groups,while the activities of T-SOD,CAT were increased;the mRNA expression of TGF-β1mRNA were decreased,GSH-Px was increased in rutin high-concentration,medium-concentration groups,there was statistical significance（P〈0.01,P〈0.05）. CONCLUSIONS：Rutin can protect mesangial cells of high glucose model rats via TGF-β1/Smad pathway,and is a potent protective agent against diabetic nephropathy.