RP-HPLC法同时测定复方美托洛尔尼群地平胶囊中3种组分含量

目的:建立同时测定复方美托洛尔尼群地平胶囊中3种组分(氢氯噻嗪、酒石酸美托洛尔、尼群地平)含量的方法。方法:采用反相高效液相色谱法。色谱柱为Agilent ZORBAX Eclipse XDB-C18,流动相为醋酸盐缓冲液-乙腈,梯度洗脱,流速为2.0 ml/min,柱温为30℃,检测波长为275 nm。结果:氢氯噻嗪、酒石酸美托洛尔、尼群地平检测质量浓度线性范围分别为45.8⁶87、92.4687、92.4¹ 386、46.11 386、46.1⁶92μg/ml(r均为0.999 9);平均加样回收率分别为100.27%、100.51%、99.42%,RSD分别为0.69%、1.06%、0.90%(n=6)。结论:该方法简便、准确、重复性好,可为复方美托洛尔尼群地平胶囊质量标准的改进提供参考。

Simultaneous Determination of 3 Components in Compound Metoprolol Nitrendipine Capsules by RP- HPLC

OBJECTIVE ： To establish a method for the content determination of hydrochlorothiazide, metoprolol tartrate and nitrendipine in Compound metoprolol nitrendipine capsules. METHODS： RP-HPLC method was adopted. The determination was performed on Agilent ZORBAX Eclipse XDB-C18 column with mobile phase consisted of acetate buffer-acetonitrile （gradient elution） at the flow rate of 2.0 ml/min. The column temperature was 30 ℃, and the detection wavelength was set at 275 nm. RESULTS： The linear ranges of hydrochlorothiazide, metoprolol tartrate and nitrendipine were 45.8-687 μg/ml （r=0.999 9）, 92.4-1 386 μg/ml （r=0.999 9） and 46.1-692 μg/ml （r=0.999 9）, respectively. The average recoveries were 100.27% （RSD=0.69% , n=6）, 100.51% （RSD=1.06%, n=6） and 99.42% （RSD=0.90%, n=6）, respectively. CONCLUSIONS： The method is sensitive, accurate and reoroducible for the imorovement of aualitv standard of Compound metoorolol nitrendioine cansules.