胃癌组织中端粒酶活性的研究

目的探讨胃癌组织中端粒酶活性的临床意义，研究非放射性端粒重复扩增法（ＴＲＡＰ）检测端粒酶的可行性。方法用ＴＲＡＰ银染法检测端粒酶在５８例胃癌，１２例胃腺瘤和９例胃溃疡组织的表达情况。结果８４．５％（４９／５８）的胃癌组织中检测到端粒酶活性，正常粘膜对照组无１例检测到端粒酶活性（Ｐ＜０．００１），１２例胃腺瘤、９例胃溃疡组织中各有１例检测到端粒酶活性。胃癌组织端粒酶的表达与年龄、肿瘤大小、分化程度、浸润深度、淋巴结转移及ＴＮＭ分期等临床病理参数无明显相关（Ｐ＞０．０５）。结论端粒酶是一种较理想的胃癌肿瘤标志物；ＴＲＡＰ银染法是一种值得推广的检测端粒酶活性的方法。

Study of telomerase activity in gastric carcinomas

Objective To appraise the telomerase activity in human gastric carcinomas and the clinical significance and to evaluate the feasibility of nonradioisotopic TRAP assay to detect telomerase activity. Methods Telomerase activity was detected by a nonisotopic PCRbased TRAP assay (Telomeric repeat amplification protocol) in tissue samples from 58 gastric carcinomas, and their matched normal tissues, 12 gastric adenomas and 9 gastric ulcer. Results None of the normal tissues exhibited telomerase activity. In contrast, 49 of 58 gastric cancer specimens were positive for telomerase activeity with a 84.5% positive rate (P<0.001), 1/12 gastric adenomas and 1/9 gastric ulcer were also positive. The incidence of telomerase activity in gastric carcinoma tissues was not correlated to age, tumor diameter, histological grade,tumor invasion in depth, lymph node metastasis or TNM staging (P>0.05). Conclusions Telomerase activity will be a good diagnostic marker for the detection of gastric carcinoma. The nonradioisotopic TRAP assay is a method feasible to detect telomerase activity.