常见ABO抗原和稀有A2抗原同步基因分型的研究

目的建立人类ABO血型系统的同步基因分型法研究ABO血型。方法采用PCR-SSP技术,设计合成13对序列特异性引物,摸索最佳退火温度,通过调整引物浓度、Mg2+浓度等,使ABO系统等位基因在同一条件下进行同步扩增和扩增产物在同一凝胶中进行同步电泳;使用此方法,检测34例已知血清学ABO血型的与20例已知序列的A2亚型标本。结果用基因分型法检测出的ABO常见基因和A2基因,与已知血清学ABO分型结果相符合。结论该ABO基因分型方法适合中国人ABO基因常规鉴定与研究。

Study on the simultaneous genotyping of common ABO antigens and rare A2 antigen

Objective To set up the simultaneous genotyping of human ABO antigens by PCR-SSP assay,and to study on ABO blood group.Methods 13 pairs of sequence-specific primers were designed and synthesized.The annealing temperature for all the primer pairs,the concentrations of each primer pair and Mg~(2 ) were adjusted to the optimum so that ABO alleles could be amplified simultaneously under the same PCR cycling parameters.The electrophoresis products were conducted simultaneously on the same agarose gel.34 samples of predetermined ABO phenotypes and 20 samples of A2 subgroups previously sequenced were genotyped by the method.Results A concordance rate of 100 percent was observed between the results of 54 samples by PCR-SSP and that of known common ABO phenotypes and rare A2 alleles.Conclusion ABO genotyping is appropriate for the routine ABO genotyping and research for Chinese population.