垂体瘤转化基因在急性髓系白血病患者中表达的研究

本研究探讨垂体瘤转化基因（pituitarytumor-transforming gene,pttg）在急性髓系白血病（acute myeloid leukemia,AML）患者中的表达及其与AML发生的关系,以及pttg基因在FAB亚型中的表达是否存在差异性。采用实时荧光定量PCR方法检测47例AML患者及28例正常对照者骨髓中pttg基因的表达水平。结果表明：AML患者pttg基因的表达水平（1.1323±1.3934）×105]明显高于正常对照组（4.5766±1.1817）×103]（p〈0.05）,急性早幼粒细胞细胞白血病（M3）患者中pttg基因的表达水平高于其他FAB亚型,其中包括急性髓系白血病未分化型（M1）、急性粒细胞白血病部分分化型（M2）、急性粒细胞单核细胞白血病（M4）、急性单核细胞白血病（M5）。结论：pttg基因的过度表达可能与AML的发生发展有关,其中可能与M3发生的关系更加密切,本研究为AML的发病机制及其基因靶向治疗的研究提供了新的思路。

Expression of Pituitary Tumor Transforming Gene in Patients with AML

The aim of study was to explore the expression of pituitary tumor-transforming gene （pttg） in acute myeloid leukemia（AML）and its relationship with the pathogenesis of AML,simultaneously to investigate the difference of the pttg expression among AML different subtypes. The expressions of pttg mRNA were quantitatively detected by real-time fluorescence quantitative polymerase chain reaction （real-time PCR） in bone marrow from 47 patients with AML and 28 normal controls. The results indicated that the expression of pttg mRNA was significantly higher in AML patients （1.1323±1.3934）×105] than that in normal controls （4.5766±1.1817）×103] （p0.05）. The expression of pttg mRNA was higher in M3 patients than that in other AML subtypes,such as M1,M2,M4,M5. It is concluded that the overexpression of pttg may be related to the pathogenesis and progression of AML,in which the overexpression of pttg may be more intimately related to the pathogenesis and progression of M3. This study provides a new idea to research the pathogenesis and targeted gene therapy of AML.