TPN加n-3脂肪酸、1,6二磷酸果糖及谷氨酰胺对小肠移植大鼠的作用

目的：探讨ＴＰＮ加用ｎ－３脂肪酸、１，６二磷酸果糖及谷氨酰胺对小肠移植大鼠特殊营养支持的作用及可能机理。 方法：１９６只近交系Ｗｉｓｔａｒ大鼠分别作为供、受体行全小肠异位移植，术前和术后应用ｎ－３脂肪酸灌胃和ＴＰＮ中加入１，６二磷酸果糖、谷氨酰胺静脉输注１０天，测量体重变化及应用图像分析观察小肠粘膜结构改变。 结果：补充外源性ｎ－３脂肪酸、１，６－二磷酸果糖和谷氨酰胺后体重丢失减少，移植小肠绒毛高

Effects of nutritional support combined with n-3 fatty acids,fructose-1,6-diphosphate and glutamine on small bowel transplanted rats

Objectives:To investigate the effects and possible mechanism of n 3 fatty acids(n 3 FA),fructose 1,6 diphosphate(FDP) and Glutamine(Gln) on small bowel transplanted rats. Methods:196 inbred strain Wistar rats underwent heterotopic small bowel transplantation(SBT) as donors and recipients respectively.n 3 FA,FDP and Gln were given via gastrogavage as well as venous infusion within total parenteral nutrition(TPN) solution for 10 days before and after surgery.Measurement of body weight was performed and image cytometry was used for analysing the changes of small bowel mucosal structure. Results:The loss of body weight was decreased,and the villous height,width,surface area,crypt depth and mucosal thickness were significantly increased after the supplement of exogenous n 3 FA,FDP and Gln. Conclusions:n 3 FA,FDP and Gln specific nutritional support had potential utility of maintaining nitrogen balance and could significantly improve the mucosal structure of small bowel graft.