AcSDKP对大鼠心成纤维细胞MMP-2、MMP-9活性和MMP-1表达的影响

目的探讨N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸(AcSDKP)对10%血清和血小板源性生长因子(PDGF)诱导的大鼠心成纤维细胞MMP-2、MMP-9活性和MMP-1表达的调节作用。方法明胶酶谱法检测心成纤维细胞MMP-2、MMP-9的活性。Western blot法检测心成纤维细胞MMP-1的表达。结果10%血清和PDGF使心成纤维细胞MMP-2、MMP-9活性增强,也促进MMP-1的表达;AcSDKP能够进一步增加由10%血清和PDGF诱导的心成纤维细胞MMP-2、MMP-9的活性,并促进MMP-1的表达。结论AcSDKP上调了由PDGF介导的心成纤维细胞MMPs活性或表达,这可能与AcSDKP抗心肌纤维化的作用相关。

Effect of AcSDKP on the expression of MMP-1 and activities of MMP-2 and MMP-9 in cultured cardiac fibroblasts

Objective To investigate whether AcSDKP can regulate MMP-2 and MMP-9 activity and MMP-1 expression in cultured rat cardiac fibroblasts mediated by 10?tal bovine serum and PDGF.Methods MMP-2 and MMP-9 were measured with zymography assays.The MMP-1 expression was detected with Western blot.Results10% fetal bovine serum(FBS) and PDGF stimulated MMP-2 and MMP-9 activity and MMP-1 expression of cardiac fibroblasts;AcSDKP further increased MMP-2 and MMP-9 activity and MMP-1 expression of cardiac fibroblasts induced by 10% FBS and PDGF.Conclusion AcSDKP can up-regulate MMPs activity or expression of cardiac fibroblasts mediated by PDGF,which is possibly related with the effect of AcSDKP anti-fibrosis.