| HPV感染阳性角朊细胞的原代培养及生物学特性研究 |
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| 引用本文： | 严泉剑,张菊,闫小君,段杰,张宇梅,赵锦荣,苏成芝.HPV感染阳性角朊细胞的原代培养及生物学特性研究[J].第四军医大学学报,2001,22(2):171-173. |
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| 作者姓名： | 严泉剑 张菊 闫小君 段杰 张宇梅 赵锦荣 苏成芝 |
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| 作者单位： | 第四军医大学全军基因诊断研究所, |
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| 摘 要： | 目的 培养人原代乳头瘤病毒（ＨＰＶ）感染阳性皮肤角朊细胞，观察其生物学特性。方法 取患者新鲜疣体及正常包皮皮肤组织，用胰酶消化法培养角朊细胞，观察其生长情况，绘制细胞生长曲线图，用ＰＣＲ及原位杂交方法检测上述细胞。结果 ＨＰＶ阳性角朊细胞（ＰＫ）在１６４０液中生长情况良好，增殖速度较正常角朊细胞快。结论 ＰＫ细胞在１６４０液中生长情况良好，适合作为ＨＰＶ感染动物模型用于进一步研究。
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| 关 键 词： | 人乳头瘤病毒感染 角朊细胞 原代培养 生物学特性 |
| 文章编号： | 1000-2790(2001)02-0171-03 |
| 修稿时间： | 1999年11月1日 |
Primary culture and biologic characters of human papillomavirus positiv e keratinocytes |
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| YAN Quan-Jian,ZHANG Ju,YAN Xiao-Jun,DUAN Jie,ZHANG Yu-Mei,ZHAO J in-Rong,SU Cheng-Zhi.Primary culture and biologic characters of human papillomavirus positiv e keratinocytes[J].Journal of the Fourth Military Medical University,2001,22(2):171-173. |
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| Authors: | YAN Quan-Jian ZHANG Ju YAN Xiao-Jun DUAN Jie ZHANG Yu-Mei ZHAO J in-Rong SU Cheng-Zhi |
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| Abstract: | AIM To investigate the biologic characters of HPV positive keratinocytes in culture. METHODS By protecting the cell growth curve, we studied the distinctness of growth character between HPV positive keratinocytes (PK) and normal keratinocytes (NK). HPV positive keratinocytes were confirmed by PCR and DNA hybridization. RESULTS PK proliferated more quickly than NK, and grew well in RPMI 1640 midium but did not form clone. CONCLUSION PK grew well in RPMI 1640 midium in which immunocell grows well, so is a suitable animal model for HPV infection. |
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| Keywords: | papillomavirus human keratinocytes cells culture biologic characters |
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