p63基因和蛋白在卵巢浆液性肿瘤中的表达

 目的 探讨p63基因和蛋白在卵巢浆液性肿瘤中的表达及其与卵巢恶性肿瘤发生、发展的关系。方法 采用RT-PCR法、原位杂交法和免疫组化法检测正常卵巢及卵巢良性、交界性、恶性浆液性肿瘤组织中p63基因及蛋白表达水平的变化。结果　经RT-PCR和免疫组化方法检测，p63在正常卵巢组织中表达较低；在良性肿瘤组织、交界性肿瘤组织、恶性浆液性肿瘤组织中表达水平逐渐增高。原位杂交实验结果显示，在38例卵巢癌标本中p63基因阳性表达率为92.1 %（35／38），在卵巢交界性肿瘤中阳性表达率为50.0 %（10／20），在正常卵巢组织中不表达（0／20）。结论 p63在卵巢组织中的表达与肿瘤的进展密切相关；随组织学分期和临床病理分期不同，p63基因及蛋白水平表达不同。

Expression and function relations of p63 in ovarian serous carcinoma

Objective To study the expression and function relations of the p63 in ovarian serous carcinoma, and compare them with normal ovary tissue. Methods RT- PCR, in situ hybridization and im－munohistochemistry were used to detect the expression of the p63 in ovarian serous carcinoma, ovarian bor－derline serous tumors, ovarian benign serous tumors and normal ovary tissues. Results All samples ex－pressed p63 mRNA through RT-PCR and immunohistochemistry, while the levels of expression in ovarian tu－mors was higher than that of normal ovary. p63 in human borderline and malignant ovarian tumors was higher than that in human benign ovarian tumors with significant difference, which suggested p63 may play great roles in the origin and progression of ovarian tumors. The result of in situ hybridization showed that positive expression rate of p63 mRNA in ovarian cancer (92.1 %) was significantly higher than that in normal ovary(0) or LWP ovarian cancer(50.0 %). Conclusion Expression of p63 plays an important role in the development of varian carcinoma, and along with the difference of histological stage and clinico-pathological stage, the ex－pression of p63 gene and protein level were different.