内毒素休克狗白细胞糖皮质激素受体的变化

本文研究了内毒素休克时狗白细胞糖皮质激素受体(GCR)的变化。以粘质沙雷菌内毒素复制内毒素休克模型。分别于内毒素注射前、注射后2小时、6小时取血,分离白细胞,以[~3H]-地塞米松(~3H-Dex)为放射性配基,测定了白细胞特异结合(Rs)的变化。两组动物(麻醉和清醒)实验结果表明。内毒素注射后2小时白细胞Rs均显著降低(P<0.01,P<0.05),6小时均有恢复的趋势。在清醒组,内毒素注射后6小时GCR结合容量不变,平衡解离常数显著增高(P<0.01)。提示GCR的亲和力下降。本研究结果提示:糖皮质激素在受体水平上作用的变化对内毒素休克的发生发展可能起着一定的作用。

Changes in canine leukocytic glucocorticoid receptors during endotoxin shock

We studied the changes in canine leukocytic glucocorticoid receptors during endotoxin shock. Blood samples for analysis were collected and leukocytes were isolated Just prior to, 2 and 6 hours after endotoxin administration. Employing ~sH-Dexamethasone (~3H-Dex) as a ligand, we studied ~3H-Dex specific binding of the leukocytes in dogs and their changes during endotoxin shock. Results from two groups (anesthetized and conscious) showed that the specific binding of the leukocytes decreased significantly in both groups 2 hours after endotoxin administration, but they tended to return to normal level by 6 hours. We also studied the changes of GCRS after cndotoxin injection by Scatchard analysis in conscious dogs. The apparent binding capacity (Ro) seems to unchange but the apparent equilibrium dissociation const ant (Kd) increased significantly 6 hours after endotoxin injection, suggesting that the affinity of GCRs decreased. It may be suggested that perturbations in glucocorticoid hormone action at the receptor level might bc involved in the pathogenesis of cndotoxin shock.