Bcl-2和Bax基因产物在小鼠柯萨奇病毒性心肌炎心肌组织中的表达

目的　探讨Bc1 2和Bax基因在病毒性心肌炎进展中的作用。方法　柯萨奇病毒B3 (CVB3 )感染12 5只雄性Balb/c小鼠 ,分别于d7、10、14、2 1、2 8随机取 2 0只实验组小鼠和 5只对照组小鼠处死 ,采用免疫组织化学方法检测CVB3 病毒性心肌炎小鼠不同时期心肌组织中Bcl 2和Bax蛋白表达情况。结果　病毒性心肌炎发病率为 86 % ,其中 80 %小鼠为轻、中度病变。正常对照组心肌中 ,无Bc1 2蛋白表达。在实验组中Bc1 2蛋白表达的动态变化与心肌病变积分呈显著正相关 (r =0 .93　P <0 .0 1) ,感染后 7～ 14d ,实验组小鼠心肌组织中Bax蛋白表达显著高于正常对照组 (P <0 .0 5 )。少数心肌浸润淋巴细胞表达Bc1 2和Bax。结论　Bc1 2和Bax基因参与病毒性心肌炎细胞凋亡的调控。

Expression of Bcl-2 and Bax gene production in mice with coxsakievirus myocarditis

Objective To investigate the role of Bcl 2 and Bax gene in the development of viral myocarditis(VM).Methods The total of 125 Balb/c mice were used in the experiment. Twenty mice in each experimental group (25 total in each group) were inoculated with 10 9 Tcid 50 CVB 3 0.12 ml and 5 mice (control) inoculated with saline. These mice were sacrificed on 7,10,14,21,28 days respectively after inoculated. Presence of apoptosis related proteins Bcl 2 and Bax in myocardium was determined by immunohistochemistry.Results The gross incidence of VM in the experiment group was 86 %.About 80 % of mice with VM showed mild to moderate histopathology. The dynamic changes of Bcl 2 expression level in experimental group showed significant positive correlation with the changes of myocardial histopathologic scores ( r=0.93 P <0.01).The amount of Bax protein increased prominently in the myocardium from experimental groups compared with control group( P <0.05). The infiltrating lymphocytes in the myocardium also expressed Bcl 2 and Bax.Conclusion Bcl 2 and Bax gene were involved in the regulation of apoptosis in VM