| 大鼠脑缺血再灌流脑区一氧化氮变化的研究 |
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| 引用本文： | 张洪,慕容慎行.大鼠脑缺血再灌流脑区一氧化氮变化的研究[J].临床神经病学杂志,1999,12(1):18-20. |
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| 作者姓名： | 张洪 慕容慎行 |
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| 作者单位： | 福建医科大学附属第一医院福建省神经病学研究所，福建医科大学生化教研室 |
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| 摘 要： | 目的研究大鼠脑缺血及再灌流后脑部一氧化氮的变化。方法采用荧光法和放射免疫法测定４个脑区一氧化氮（ＮＯ）代谢产物ＮＯ２和环磷酸鸟苷（ｃＧＭＰ）。结果脑缺血１０ｍｉｎ，各脑区ＮＯ２和ｃＧＭＰ含量明显增高；脑缺血３０ｍｉｎ，各脑区ＮＯ２和ｃＧＭＰ含量开始下降。缺血１０ｍｉｎ再灌流１５ｍｉｎ以及缺血３０ｍｉｎ再灌流１５ｍｉｎ，各脑区ＮＯ２和ｃＧＭＰ含量再次增加，与单纯脑缺血组相比，有显著差异性（Ｐ＜０．０５或Ｐ＜０．０１）。结论ＮＯ参与了脑缺血再灌流的损伤过程
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| 关 键 词： | 脑缺血 再灌流 一氧化氮 环磷酸鸟苷 病理 |
Change of nitric oxide in rat brain regions after cerebral ischemia reperfusion |
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| Zhang Hong,Murong Shengxing,Liu Youyao,et al..Change of nitric oxide in rat brain regions after cerebral ischemia reperfusion[J].Journal of Clinical Neurology,1999,12(1):18-20. |
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| Authors: | Zhang Hong Murong Shengxing Liu Youyao |
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| Institution: | Zhang Hong,Murong Shengxing,Liu Youyao,et al.Institute of Neurology,the First Affiliated Hospital of Fujian Medical University,Fuzhou 350005 |
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| Abstract: | Objective To study the change of nitric oxide (NO) in rat cerebral cortex,hippocampus,striatum and cerebellum after cerebral ischemia reperfusion(IR). Methods NO production nitrite(NO 2) and cyclic guanosine monophosphate(cGMP) in four brain regions were measured with fluorometric method and radioimmunoassay. Results Concentrations of NO 2 and cGMP in brain regions were significantly higher at 10 minutes interval and gradually decreased at 30 minutes interval after ischemia . The amount of NO 2 and cGMP in brain regions at 10 minutes ischemia followed by 15 minutes reperfusion group and 30 mi nutes ischemia followed by 15 minutes reperfusion group were significantly higher than those at 10 minutes ischemia group and 30 minutes ischemia group respectively ( P <0.05 or P <0.01). Conclusion NO is involved in the pathogenesis of cerebral ischemia reperfusion. |
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| Keywords: | Cerebral ischemia Reperfusion Nitric oxide Cyclic guanosine monophosphate |
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