| 蒙药小白蒿野生品与栽培品中绿原酸的含量比较研究 |
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| 引用本文： | 智慧,艾丹,杨秀华,刘佳,斯琴格日乐,张玲玲. 蒙药小白蒿野生品与栽培品中绿原酸的含量比较研究[J]. 中国民族医药杂志, 2020, 0(5): 40-43 |
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| 作者姓名： | 智慧 艾丹 杨秀华 刘佳 斯琴格日乐 张玲玲 |
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| 作者单位： | 呼伦贝尔职业技术学院 |
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| 基金项目： | 内蒙自治区高等学校科学研究项目(编号:NJZY18353)。 |
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| 摘 要： | 目的:测定小白蒿野生品与栽培品中绿原酸的含量,为蒙药常用药材小白蒿的合理开发与规范化栽培提供理论依据。方法:采用高效液相色谱(HPLC)法测定野生品与栽培品的小白蒿中绿原酸含量,色谱条件:C18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈:0.4%磷酸溶液(12∶88);流速:1.0 mL/min;柱温:30℃;检测波长为327 nm;进样量:10μL。结果:测定野生品和栽培品小白蒿中绿原酸的含量分别为1.4485mg/g、0.9550 mg/g。结论:该方法测定小白蒿中绿原酸含量操作简便、重现性好,野生品与栽培品的绿原酸含量相当,有利于蒙药小白蒿的驯化和资源保护。
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| 关 键 词： | 小白蒿 绿原酸 HPLC 含量测定 |
A comparative study on the content of chlorogenic acid in wild and cultivated Artemisia frigida Willd |
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| ZHI Hui,AI Dan,YANG Xiu-Hua,LIU Jia,Siqingerile,ZHANG Ling-ling. A comparative study on the content of chlorogenic acid in wild and cultivated Artemisia frigida Willd[J]. Journal of Medicine & Pharmacy of Chinese Minorities, 2020, 0(5): 40-43 |
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| Authors: | ZHI Hui AI Dan YANG Xiu-Hua LIU Jia Siqingerile ZHANG Ling-ling |
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| Affiliation: | (Hulunbeier Vocational Technical College,Hu lun buir 021000) |
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| Abstract: | Objective:To determine the content of Chlorogenic acid in wild products and cultivated products of Artemisia frigida Willd and provide a certain theoretical basis for the rational development and standardized cultivation of Mongolian medicinal materials Artemisia frigida Willd.Methods:High-performance liquid chromatography(HPLC)method was used to determine the content of Chlorogenic acid in wild and cultivated Artemisia frigida Willd.0.4%phosphoric acid solution(12∶88);flow rate:1.0 mL/min;column temperature:30℃;detection wavelength is 327 nm;injection volume:10μL.Results:The content of Chlorogenic acid in wild products and cultivated products Artemisia frigida Willd was determined,which were 1.4485 mg/g,0.9550 mg/g.Conclusion:This method is simple and easy to operate to determine the content of Chlorogenic acid in Artemisia annua.Artemisia frigida Willd domestication and resource protection. |
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| Keywords: | Artemisia frigida willd chlorogenic acid HPLC determination of content |
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