橙汁提取物生物制备银纳米颗粒及其生物相容性研究

目的探索橙汁提取物生物制备银纳米颗粒产量最大化的条件;评价其银纳米颗粒的生物相容性。方法检测反应体系在不同pH、温度与反应时间等条件下银纳米颗粒产量变化,探索最佳反应条件;通过CCK-8试剂盒、扫描电镜和抑菌实验评价银纳米颗粒的生物相容性及抗菌性。结果在pH=8.0、0℃、反应时间为4 h条件下银纳米颗粒产量最高。制备的银纳米颗粒呈球形或椭球形,平均粒径为15.54 nm,Zeta电位为-34.46,分散良好。浓度介于6.25~50μg/ml的银纳米颗粒对大鼠皮肤成纤维细胞和红细胞均无毒性作用。在12.5μg/ml及以上浓度银纳米颗粒对大肠杆菌具有抑制作用,100μg/ml及以下浓度银纳米颗粒对金黄色葡萄球菌未见明显抑制作用。结论在pH=8.0、0℃、反应时间为4 h条件下利用橙汁提取物制备银纳米颗粒产量最高,这种银纳米颗粒粒径小、分散均匀、不易团聚,具有良好的生物相容性和抗菌性。

Study on the biosynthesized silver nanoparticles using extractive orange juice and their biocompatibility

Objective　To explore the conditions for maximizing the yield of silver nanoparticles synthesized by extractive orange juice, and to evaluate the biocompatibility of such silver nanoparticles. Methods　The yield of silver nanoparticles was measured under different pH values, temperature and reaction time, and the optimal reaction conditions were explored. The biocompatibility and antibacterial properties of silver nanoparticles were evaluated by CCK-8 kit, scanning electron microscopy and antibacterial experiments. 　Results 　When the pH value was 8.0, temperature was 0 ℃, reaction time was 4 h, the yield of silver nanoparticles was highest. The synthesized silver nanoparticles were spherical or ellipsoidal, with an average particle size of 15.54 nm and a zeta potential of -34.46, which was well dispersed. Silver nanoparticles with a concentration between 6.25-50 μg/ml had no toxic effects on rat skin fibroblasts and red blood cells. Silver nanoparticles at 12.5 μg/ml and above had significant inhibitory effects on E. coli, and silver nanoparticles at 100 μg/ml and below did not significantly inhibit S. aureus.　Conclusions　The silver nanoparticles synthesized by the extractive orange juice at pH=8.0, temperature of 0 ℃ and reaction time of 4 h have the highest yield. These silver nanoparticles have small particle size, uniform dispersion, and are not easy to agglomerate, and have good biocompatibility and antibacterial properties.