TNIK在胃癌组织中的表达及其临床意义

目的探讨肿瘤坏死因子受体相关因子-2和Nck相互作用蛋白激酶(Traf-2 and Nck-interacting protein kinase,TNIK)在胃癌组织中的表达及其临床意义。方法回顾性收集2014年7月至2017年12月期间在郑州大学附属洛阳中心医院普通外科行手术切除并经病理科确诊的胃癌组织及其癌旁组织标本78份,以及同期胃溃疡组织标本42份。采用免疫组织化学染色法及Western blot法检测上述3种组织中TNIK蛋白的表达情况,并探索TNIK蛋白表达与胃癌临床病理学特征及预后的关系。结果免疫组织化学染色结果显示,胃癌组中,TNIK蛋白的阳性表达率为66.67%(52/78),癌旁组织组为11.54%(9/78),胃溃疡组为21.43%(9/42)。胃癌组的TNIK蛋白阳性表达率高于癌旁组织组和胃溃疡组(P<0.05),但癌旁组织组和胃溃疡组比较差异无统计学意义(P>0.05)。Western blot结果显示,在胃癌组中,TNIK蛋白的表达水平高于胃溃疡组及癌旁组织组,差异均有统计学意义(P<0.05);但癌旁组织组与胃溃疡组比较差异无统计学意义(P=0.772)。TNIK蛋白的表达与胃癌患者的性别、CEA值、肿瘤直径及病理学类型均无关(P>0.05),但与胃癌患者的年龄、TNM分期、分化程度、远处转移及淋巴结转移相关(P<0.05);TNIK蛋白阳性表达患者的预后差于阴性表达者(P<0.001)。结论TNIK蛋白在胃癌组织中高表达,且与胃癌预后不良因素及较差的预后相关,可能是潜在的胃癌诊治靶点。

Expression and significance of Traf-2 and Nck-interacting kinase in gastric cancer tissues

Objective To investigate the expression level and the clinical significance of Traf-2 and Nckinteracting protein kinase(TNIK)in gastric cancer tissues.Methods We retrospectively collected 78 cases of gastric cancer and its adjacent tissues which were diagnosed by surgery and pathology,all the patients received surgery in the Department of General Surgery in Luoyang Central Hospital Affiliated to Zhengzhou University,from July 2014 to December 2017,as well as 42 cases of gastric ulcer tissues in the same period.The expression of TNIK protein was detected by using immunohistochemical method and Western blot method in 78 cases of human gastric cancer tissues,corresponding adjacent normal tissues,and 42 cases of gastric ulcer tissues,the relationship between expression of TNIK protein and clinicopathologial features of gastric cancer was explored.Results The positive expression rate of TNIK protein in gastric cancer tissues was 66.67%(52/78),and adjacent cancer tissues showed low expression rate of 11.54%(9/78),while it was 21.43%(9/42)in gastric ulcer tissues.On the positive expression rate of TNIK protein,gastric cancer tissues compared with adjacent cancer tissues and gastric ulcer tissues,the difference was statistically significant(P<0.05),however,there was no statistically significant difference between adjacent cancer tissues and gastric ulcer tissues(P>0.05).Western blot method showed that the expression level of TNIK protein in gastric cancer tissues was all significantly higher than that in adjacent cancer tissues and gastric ulcer tissues,the differences were statistically significant(P<0.05),but the difference was not significant when compared the adjacent cancer tissues and gastric ulcer tissues(P=0.772).The expression of TNIK protein was irrelevent to sexual distinction,CEA value,tumor diameter,and pathological type(P>0.05),while it was closely related to patients’age,TNM stage,differentiation grade,distant metastasis,and lymph node metastasis(P<0.05).The prognosis of patients with positive expression of TNIK protein was worse than that of patients with negative expression of TNIK protein(P<0.05).Conclusion TNIK protein is highly expressed in gastric cancer tissues,which is associated with poorly prognostic factors and poor prognosis,and it may be a therapeutic target in patients with gastric cancer.