促结缔组织增生型成釉细胞瘤的组织发生与细胞分化机制探讨

目的：探讨促结缔组织增生型成釉细胞瘤（desmoplastic ameloblastoma,DA)的组织发生与细胞分化机制。方法：对20例DA中不同类型的蛋白细丝的表达进行免疫组化检测，并对4例DA进行超微结构观察。结果：免疫组化标记表明，肿瘤上皮中梭形、多边形细胞CKH、CK8、CK19呈不同程度阳性，间质中纤维细胞vimentin阳性，部分间质细胞smooth muscle actin阳性。超微结构观察见肿瘤上皮细胞内存在张力丝，细胞间桥粒连接，间质中见肌纤维母细胞，部分上皮巢周围组织疏松。结论：DA可能为牙胚发生较早阶段发生的肿瘤，且肿瘤上皮有向角化细胞分化的倾向。

Histogenesis and celldifferentiation of desmoplastic ameloblastoma

Objective:To study the histogenesis and cell differentiation of desmoplastic ameloblastoma.Methods:The expression of various protein filaments was detected by immunohistochemical stainning in 20 cases of desmoplastic ameloblastoma and the ultrastructure of the tumor was observed in 4 cases.Results:CKH,CK8 and CK19 were positive in spindle-shaped and polygonal epithlium tumor cells.Vimentin was positive in mesenchymal fibrocytes and smooth muscle actin in some mesenchymal cells.Ultrastructural observation found that tonifilaments were in epithelial cells and there were desmosomes between these cells.In stroma myofibroblasts were presented.Loose connective tissue could be seen around some epithelial cell nests.Conclusion:Desmoplastic ameloblastoma may be arise from the early stage of the dental germ formation, epithelial cells of the tumor had the tendency to differentiate to the keratinized cells.