The Defect in the Hurler and Scheie Syndromes: Deficiency of α-L-Iduronidase |
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Authors: | Gideon Bach Robert Friedman Bernard Weissmann Elizabeth F. Neufeld |
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Affiliation: | .The National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda, Maryland 20014;.Department of Biochemistry, University of Illinois, College of Medicine, Chicago, Ill. 60612 |
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Abstract: | Skin fibroblasts cultured from patients affected with the Hurler or Scheie syndromes (mucopoly-saccharidoses I or V, respectively) have a functional deficiency of a protein required for catabolism of sulfated mucopolysaccharide that has been designated the "Hurler corrective factor." We now show Hurler factor purified from normal human urine to be associated with alpha-L-iduronidase activity. Cell lines deficient in Hurler corrective factor have no detectable activity of alpha-L-iduronidase (less than 3% of that found in cells from individuals of other genotypes). Such correspondence indicates that Hurler corrective factor and alpha-L-iduronidase are the same entity. Correction of deficient cells is accompanied by an efficient uptake of alpha-L-iduronidase from the medium. |
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