毛细管电泳-激光诱导荧光分离检测手性5-(2-氨基丙基)-2-甲氧基苯磺酰胺对映体

目的建立毛细管电泳——激光诱导荧光法分离测定(R/S)-5-(2-氨基丙基)-2-甲氧基苯磺酰胺对映体。方法用异硫氰酸荧光素衍生(R/S)-5-(2-氨基丙基)-2-甲氧基苯磺酰胺,衍生产物在70mmol/L的硼砂(pH=9.5)缓冲液中用毛细管电泳分离,激光诱导荧光方法测定。结果(R/S)-5-(2-氨基丙基)-2-甲氧基苯磺酰胺对映体得到了基线分离,线性范围均为8.20×10-8～9.02×10-7mol/L。结论本方法简单,精密度高,灵敏度高,可为盐酸坦索罗辛的质量控制提供方法。

Capillary electrophoresis for chiral separation and laser-induced fluorescence detection of 5-(2-aminopropyl)-2-methoxybenzenesulfonamide enantiomers

Objective To establish an HPCE-LIF method for separation and determination of the intermediate of tamsulosin hydrochloride, (R/S)-5-(2-aminopropyl)-2-methoxybenzenesulfonamide (R/S-APMB). Methods (R)-5-(2-aminopropyl)-2-methoxybenzenesulfonamide reacted with fluorescein isothiocyanate (FITC) and then FITC-R-APMB was obtained. FITC-R-APMB was separated using 70 mmol/L borate as a buffer (pH=7.5). The 47 cm uncoated fused-silica capillary with 75 μm internal diameter (effective length 40 cm) was used. The capillary temperature was maintained at 20 ℃. Samples were injected at a pressure of 3.45 kPa in 1 s and separated at 20 kV. Results Both R- and S-APMB had low detection limits (8.2×10-9 mol/L). The linear range of R- APMB was 8.20×10-8～9.02×10-7 mol/L. Conclusion The HPCE-LIF method may offer a simple and highly sensitive approach for quality control of tamsulosin production.