湖北地区宫颈疾病患者HPV基因型及其年龄分布

目的:分析湖北地区宫颈疾病患者人乳头状瘤病毒(HPV)感染的基因型分布及与年龄的关系。方法:4 314例宫颈疾病患者,年龄21-86岁,按5岁一组分为10组,收集各组宫颈脱落细胞样本,应用PCR-基因芯片技术进行HPV分型检测,统计分析各组HPV总感染率和各亚型感染率及其年龄分布特点。结果:HPV总感染率为28.86%(1 245/4 314)。以单纯高危型HPV(hrHPV)为主,感染率为23.78%(1 026/4 314),单纯低危型HPV(lrHPV)感染率只占2.30%(99/4 314),余为混合感染。高危型中又以HPV16型最多,感染率为9.23%(398/4 314),其次为HPV52和58型,感染率为5.42%(234/4 314)和4.75%(205/4 314)。HPV总感染率以21-25岁组(29.97%)和51-55岁组(31.75%)最高,最低为31-35岁组(25.19%)和65岁组(25.42%),但各组间差异无显著性(P0.05)。单一感染率明显较多重感染率高(20.91%vs 7.95%,P0.01)。结论:加强湖北地区宫颈疾病患者HPV感染的筛查和监测,有利于该地区女性人群生殖器疣和宫颈癌防治以及流行病学研究。

Investigation and Analysis of HPV Infection Status in Patients with Gynecological Disease in Hubei Province

Objective:To investigate the distribution of different human papillomavirus(HPV)subtypes infection in patients with gynecological disease in Hubei province.Method:A total of 4 314 cases were recruited and the cervical cell samples were collected for detection for HPV genotypes by using PCR and gene chip.Results:1 245 out of 4 314 cases were detected with HPV-positive infection(28.86%,1 245/4 314),1026 cases were detected with only high risk HPV(hrHPV)infection(23.78%,1026/4 314),99 cases were detected with only low risk HPV(lrHPV)infection(2.30%,99/4 314)and the rest were detected with both hrHPV and lrHPV infection.The most prevalence hrHPV subtype were HPV16(9.23%,398/4 314),HPV52(5.42%,234/4 314)and HPV58(4.75%,205/4 314).The 21-25group(29.97%)and 51-55group(31.75%)had high prevalence while 31-35group(25.19%)and >65group had low prevalence.But there was no significant difference(P>0.05)between two groups.Single HPV type infection rate was significantly higher than multiple HPV type infection rate(20.91%vs 7.95%,P<0.01).Conclusion:It is necessary to screen HPV for prevention of genital warts and cervical cancer in Hubei region.