姜黄素对氧化应激损伤后海马神经元血红素加氧酶-1 的表达影响

目的：探讨姜黄素对氧化应激损伤后的海马神经元血红素加氧酶-1（hemeoxygenase-1，HO-1）的表达影响。方法i将培养的原代海马神经元，分为6组：空白对照组、损伤对照组、姜黄素不同剂量治疗组（2．5μmol／L，5μmol／L，10μmol／L，20μmol／L）。利用1mmol／L的H2O2处理海马神经元1h，制作神经元氧化应激损伤模型。按照以上分组情况加入等浓度的姜黄素培养液，37℃、5％CO2孵箱孵育6h，采用倒置相差显微镜观察细胞的形态变化；免疫荧光、PCR半定量检测细胞内HO-1的表达。结果：①5μmol／L组、10μmol／L组细胞形态变化不明显；损伤对照组和2．5μnol／L组细胞损伤明显；②损伤对照组与空白对照组比较，P〈0．05；损伤对照组与2．5μmol／L组、20μmol／L组两两比较差异无统计学意义（P〉0．05）；5μmol／L组、10μmol／L组与损伤对照组比较，差异有统计学意义（P〈0．05）；PCR检测结果提示20μmol／L组比2．5μmol／L组HO-1的表达量减少。结论：姜黄素对氧化应激损伤海马神经元的保护机制可能是通过上调HO-1的表达实现的。

Effect of curcumin on expression of heme oxygenase-1 oxidative stress injured hippocampal neurons

Objective： To explore the effect of curcumin on heme oxygenase-1 expression in hippocampal neurons following oxidative stress. Methods： The primary cultured hippocampal neurons were divided into 6 groups ：the blank group, injury group, and 4 different doses of curcumin treatment groups （2.5 μmol/L, 5 μmol/L , 10 μmol/L and 20 μ mol/L groups）. Oxidative stress model was established by incubating hippocampal neurons with H2O2 （1 retool/L） for 1 hour. Different levels of curcumin were added to oxidative damaged neurons and then these neurons were cultured in incubator for 6 hours. The morphology of neurons were observed and the expression of HO-! were detected by immunofluorescence and PCR. Results： （1）Neuron morphology in 5 p, mol/L and 10μmol/L group had no evident alteration.Axons of neuron in injury group and 2.5 μmol/L group were injured obviously. （2）Compared injury group with control group, the difference of expression of HO-1 was significant （P 〈 0.05）. No significant difference was observed among injury group, 2.5 μmol/L group,and 20 μmol/L group （P 〈 0.05）. HO-1 expression in 5 μmol/L group and 10 μmol/L group were strikingly enhanced （P 〈 0.05）. There was no difference of HO-1 expression among 20 μmol/L group, injury group and 2.5 μmol/L group （P 〉 0.05）. Conclusion： The protective effects of curcumin on oxidative stress in hippcampal neurons maight be through the up-regulation of HO-1.