韦格纳肉芽肿病患者树突状细胞蛋白酶活化受体-2的表达

目的 探索蛋白酶活化受体-2(PAR-2)在韦格纳肉芽肿病(WG)患者外周血免疫细胞特别是树突状细胞(DC)上的表达有无异常.方法 流式细胞仪检测健康人、活动期、非活动期、各治疗组WG患者外周血各免疫细胞、DC及DC样单核细胞表面PAR-2蛋白的表达.采用双尾Mann Whitney非参数t检验.结果 PAR-2在WG患者外周血中性粒细胞(PMN)、单核细胞、淋巴细胞等免疫细胞及DC上的表达与健康人差异无统计学意义,但在DC样单核细胞CD14+CD16+(t=3.823,P=0.004)和CD64+CD16+(t=2.652,P=0.024)单核细胞上的表达显著高于健康人.活动期WG患者外周血PMN(t=2.690,P=0.013)、单核细胞(t=1.668,P=0.047)、淋巴细胞(t=1.742,P=0.038)、BDCA3+DC(t=2.582,P=0.016)、CD11c+DC(t=1.828,P=0.044)和CD14+CD16+(t=3.419,P=0.002)和CD64+CD16+(t=3.494,P=0.005)单核细胞上PAR-2的表达显著高于非活动期患者.环磷酰胺和甲氨蝶呤治疗组WG患者上述细胞PAR-2的表达差异无统计学意义.结论 PAR-2在DC、DC样单核细胞及免疫细胞的表达与WG的发病发展相关.不同药物对PAR-2表达的影响差异无统计学意义.

Abstract：

Objective The aim of this study was to assess the status of protease-activated receptor-2(PAR-2) expression on the peripheral blood immune cells including dendritic cells(DC) of Wegener's granulo matosis(WG) patients.Methods Flow cytometry was used to analyze the expression of PAR-2 protein on peripheral blood immune cells,DC and DC-like monocytes of healthy controls (HC),inactive,active and different medicine-treated WG patients.Two-tail Mann Whitney non-parametric test was used for statistical analysis.Results There was no difference of PAR-2 expression on PMN,monocytes,lymphocytes and DC between WG patients and HC.However,PAR-2 expression on CD14+CD16+ (t=3.823,P=0.004) and CD64+CD16+ (t=2.652,P=0.024) DC-like monocytes was much higher in WG patients compared with HC.In active WG,expression of PAR-2 was up-regulated on the cell surface of PMN (t=2.690,P=0.013 ),monocytes (t=1.688,P=0.047),lymphocytes(t=1.742,P=0.038),BDCA3+DC(t=2.582,P=0.016),CD11c+DC(t=1.828,P=0.044),CD14+CD16+ (t=3.419,P=0.002)and CD64+CD16+ (t=3.494,P=0.005) DC-like monocytes when compared with inactive WG.PAR-2 expression on these cells was similar between cyclophosphamide (Cyc) and Methotrexate (MTX) treated WG patients.Conclusion PAR-2 expression on immune cells,DC and DC-like monocytes correlates with WG occurrence and development.There is no difference in the effects on PAR-2 expression between various medications.

Protease-activated receptor-2 expression on dendritic cell of Wegener's granulomatosis patients

Objective The aim of this study was to assess the status of protease-activated receptor-2(PAR-2) expression on the peripheral blood immune cells including dendritic cells(DC) of Wegener's granulo matosis(WG) patients.Methods Flow cytometry was used to analyze the expression of PAR-2 protein on peripheral blood immune cells,DC and DC-like monocytes of healthy controls (HC),inactive,active and different medicine-treated WG patients.Two-tail Mann Whitney non-parametric test was used for statistical analysis.Results There was no difference of PAR-2 expression on PMN,monocytes,lymphocytes and DC between WG patients and HC.However,PAR-2 expression on CD14+CD16+ (t=3.823,P=0.004) and CD64+CD16+ (t=2.652,P=0.024) DC-like monocytes was much higher in WG patients compared with HC.In active WG,expression of PAR-2 was up-regulated on the cell surface of PMN (t=2.690,P=0.013 ),monocytes (t=1.688,P=0.047),lymphocytes(t=1.742,P=0.038),BDCA3+DC(t=2.582,P=0.016),CD11c+DC(t=1.828,P=0.044),CD14+CD16+ (t=3.419,P=0.002)and CD64+CD16+ (t=3.494,P=0.005) DC-like monocytes when compared with inactive WG.PAR-2 expression on these cells was similar between cyclophosphamide (Cyc) and Methotrexate (MTX) treated WG patients.Conclusion PAR-2 expression on immune cells,DC and DC-like monocytes correlates with WG occurrence and development.There is no difference in the effects on PAR-2 expression between various medications.