去铁敏预处理对移植供肝保护作用的研究

目的：探索去铁敏对移植供肝的保护作用及可能机制。方法：SD大鼠，随机分为假手术组（S组）、自体肝移植组（AT组）、自体肝移去铁敏预处理组（DP组）与自体肝移植去铁敏灌肝组（DI组）。检测各组术后24 h血清中谷丙转氨酶（ALT）、谷草转氨酶（AST）和碱性磷酸酶（ALP），观察肝组织形态学改变并检测肝组织中低氧诱导因子-1（HIF-1α）及丙二醛（MDA）的含量。结果：与S组相比，AT组、DP组和DI组术后血清ALT，AST和ALP明显升高；但DP组、DI组较AT组血清ALT，AST和ALP明显降低，肝形态异常变化减轻，肝组织中HIF-1α蛋白含量升高，MDA含量下降(P﹤0.05)。结论：在肝移植术前行去铁敏预处理或术中行去铁敏溶液灌肝，对供肝有保护作用，且前者优于后者。其机制可能与提高组织HIF-1α含量及减轻脂质过氧化有关。

The protective effect of deferoxamine preconditioning on transplanted liver

To study the protective effect of deferoxamine preconditioning on transplanted liver and its possible mechanism.Methods :SD rats are randomly divided into four groups: sham operation group(S), autologous liver transplantation group(AT), deferoxamine preconditioning AT group(DP) and deferoxamine irrigation AT group(DI).At 24 hours after operation, several factors of each group were measured respectively, including deterioration of histomorphology, serum hepatic enzymes,and hypoxia inducing factor-1（HIF-1α）and malonaldehyde（MDA）in hepatic tissue. Results:The levels of alanine aminotransferase(ALT), glutamic oxalacetic transaminase(AST) and alkaline phosphatase (ALP) in AT group, DP group and DI group were higher than those of S group.In the DP and DI groups, abnormal changes in liver morphology were milder,the level of HIF-1α in hepatic tissue was increased and MDA content was decreased (P﹤0.05),as compared to the AT group.Conclusions:Deferoxamone preconditioning of graft before liver transplantion or deferoxanine inrigation of liver during operation has protective effect on donor liver, and protective effect of the former method is better than the latter.The mechanism of this preconditioning may be related to increacing HIF-1α and reducing lipid peroxidation in hepatic tissue.