周围神经挤压伤后纤溶成分表达的变化

目的 观察大鼠坐骨神经挤压伤后纤溶成分中组织型纤溶酶原激活剂（ｔ－ＰＡ）和纤溶酶原激活剂抑制因子１（ＰＡＩ－１）蛋白表达的变化，了解ｔ－ＰＡ、ＰＡＩ－１在周围神地操作后修复过程中的作用。方法 取２１只Ｗｉｓｔａｒ大鼠，分别造成坐骨神经挤压伤模型。在术后６ｈ、１ｄ、３ｄ、７ｄ、１４和２１ｄ分别取材进行组织学、免疫组化观察。结果 术后３ｄ时神经断端回缩球处的ｔ－ＰＡ抗原呈阳性反应。７ｄ时损伤进行组织学

Changes of fibrinolytic components expression in peripheral nerves after crush injury

Objective To observe the changes of tissue type plasminogen activator(t PA) and plasminogen activator inhibitor 1 ( PAI 1) expression in adult rat sciatic nerve after crush injury, and investigate the effects of t PA and PAI 1 on peripheral nerve injury and regeneration. Methods Sciatic nerve crush injury model was set up in 21 Wister rats. Histological and immunohistochemical examination of the nerve samples were performed 6 hours, 1 day, 3 days, 7 days, 14 days, and 21 days after the operation. Results The t PA antibody was positive in the retraction bulb of the proximal segments of crushed sciatic nerve after 3 days. There was enormous proliferation of Schwann cells in the lesion area. Both t PA and PAI 1 were expressed 7 days after the injury. From then on, the expression of t PA decreased whereas the expression of PAI 1 increased gradually. Conclusions T PA and PAI 1 not only participate in the pathophysiological changes of peripheral nerves after crush injury, but have some effects on peripheral nerve repair as well.