| He—Ne激光照射对肿瘤坏死因子基因表达的调控 |
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| 引用本文： | 高美华 李武修. He—Ne激光照射对肿瘤坏死因子基因表达的调控[J]. 滨州医学院学报, 1999, 22(6): 521-525 |
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| 作者姓名： | 高美华 李武修 |
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| 作者单位： | 滨州医学院!滨州市256603 |
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| 摘 要： | 目的：探讨Ｈｅ－Ｎｅ激光对ＴＭＦ基因的调控作用。方法：用斑点杂交及Ｎｏｒｔｈｅｒｎ杂交技术观测激光对肿瘤患者ＴＮＦ基因表达的影响，采用２４ｍＷ Ｈｅ－Ｎｅ激光照射荷瘤小鼠及肿瘤口才脾区（瘤区），照射前后分别取脾细胞及外周血淋巴细胞，从中提取ＲＮＡ。通过双抗体夹心ＥＬＬＳＡ法及受体吸收试验，观测激光照射对带瘤机体ＴＮＦ受体及ＴＮＦ活性的影响。结果：带瘤机体ＴＮＦｍＲＮＡ及ＴＮＦ活性明显降低，血清及培
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| 关 键 词： | 激光 He－Ne激光 肿瘤 TNFmRNA TNFR 基因 |
THE INFLUENCE OF He Ne LASER IRRADIATION ON TNF mRNA GENE EXPRESSION |
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| Gao Meihua,Li Wuxiu,Feng Xianqi,et al. THE INFLUENCE OF He Ne LASER IRRADIATION ON TNF mRNA GENE EXPRESSION[J]. Journal of Binzhou Medical College, 1999, 22(6): 521-525 |
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| Authors: | Gao Meihua Li Wuxiu Feng Xianqi et al |
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| Abstract: | Objective:To study the influence of He-Ne laser on TNF gene expression.Methods:24mW He Ne Laser was applied to irradiate spleen region with tumor in patients and mice.Spleen cell and lymphocyte in tumor body were obtained before and after irradiation, and RNA was drawn.We used spot blot hybridization and northern blot hybridization technique to observe the effect of laser on TNF gene expression in patients with tumor.Results:TNFmRNA was lower in tumor body.After laser irradiation,TNFmRNA was signifcantly raised( P <0 01),difference was notable. Conclusion:He Ne laser may promote TNFmRNA expression. |
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| Keywords: | laser He Ne tumor TNF mRNA TNF TNFR sTNFR genes mice |
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