| 变异血红蛋白对2种基于HPLC原理的糖化血红蛋白检测系统的影响 |
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| 引用本文： | 缪怡,徐雪亮,金洁美,李水军,沈博. 变异血红蛋白对2种基于HPLC原理的糖化血红蛋白检测系统的影响[J]. 上海医学检验杂志, 2009, 0(4) |
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| 作者姓名： | 缪怡 徐雪亮 金洁美 李水军 沈博 |
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| 作者单位： | 上海市徐汇区中心医院中心实验室;上海市长宁区光华医院检验科; |
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| 摘 要： | 目的比较亲和层析-高效液相色谱(AC-HPLC)系统和离子交换-高效液相色谱(IE-HPLC)系统测定血红蛋白(Hb)结构不同的血液样本中HbAlc的结果,评价变异Hb对2种检测系统的影响。方法分别用2种HPLC系统同时检测HbA1c含量在5%~11%、Hb结构正常的血液样本和含有不同浓度胎儿Hb(HbF)的血液样本以及α-地中海贫血(α-MA)和β-地中海贫血(β-MA)患者血液样本中的HbA1c浓度。结果对于Hb结构正常、HbA1c含量在5%~11%的血液样本,2种HPLC检测系统的测定结果差异无统计学意义(P>0.05)。AC-HPLC法不受血液样本中HbF的干扰;若血液样本中HbF的含量超过8.8%,IE-HPLC法测定结果存在一定的偏倚,当HbF含量达70%时甚至无法测出结果。对于α-MA和轻型β-MA患者的血液样本,IE-HPLC法的结果明显高于AC-HPLC法(P<0.01、P<0.05),而重型β-MA患者的样本用IE-HPLC法甚至无法测得结果。结论用IE-HPLC法检测含变异Hb、HbF的样本中的HbA1c,结果可能受干扰;而用AC-HPLC法则不受干扰。
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| 关 键 词： | 糖化血红蛋白 变异血红蛋白 离子交换-高效液相色谱 亲和层析-高效液相色谱 |
Effect of hemoglobin variants on the derermination of glycohemoglobin by two high performance liquid chromatography-based methods |
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| MIAO Yi,XU Xueliang,JIN Jiemei,LI Shuijun,SHEN Bo.. Effect of hemoglobin variants on the derermination of glycohemoglobin by two high performance liquid chromatography-based methods[J]. Shanghai Journal of Medical Laboratory Sciences, 2009, 0(4) |
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| Authors: | MIAO Yi XU Xueliang JIN Jiemei LI Shuijun SHEN Bo. |
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| Affiliation: | 1.Central Laboratory;Shanghai Xuhui Central Hospital;Shanghai 200031;China;2.Department of Clinical Laboratory;Shanghai Changning Central Guanghua Hospital;Shanghai 200052;China |
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| Abstract: | Objective To compare the results of HbAlc in blood samples with various configuration of hemoglobin(Hb) by affinity chromatogragh-high performance liquid chromatography(AC-HPLC)system and ion exchange-high performance liquid chromatography(IE-HPLC)system,and evaluate the effect of glycohemoglobin variants on AC-HPLC and IE-HPLC.MethodsThe levels of HbA1c in 30 blood samples with the normal Hb configuration and the HbA1c levels were 5%-11%,the blood samples with different HbF levels and the blood samples of ... |
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| Keywords: | Glycohemoglobin Hemoglobin variants Ion exchange-high performance liquid chromatography Affinity chromatogragh-high performance liquid chromatography |
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